5T3F

hen egg-white lysozyme soaked with selenourea for 10 min

5T3F の概要

• エントリーDOI: 10.2210/pdb5t3f/pdb
• 分子名称: Lysozyme C, selenourea (3 entities in total)
• 機能のキーワード: lysozyme, selenourea, hydrolase
• 由来する生物種: Gallus gallus (Chicken)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15438.30

構造登録者

Luo, Z.,Dauter, Z. (登録日: 2016-08-25, 公開日: 2016-11-30, 最終更新日: 2024-11-13)

主引用文献

Luo, Z.
Selenourea: a convenient phasing vehicle for macromolecular X-ray crystal structures.
Sci Rep, 6:37123-37123, 2016

PubMed Abstract: Majority of novel X-ray crystal structures of proteins are currently solved using the anomalous diffraction signal provided by selenium after incorporation of selenomethionine instead of natural methionine by genetic engineering methods. However, selenium can be inserted into protein crystals in the form of selenourea (SeC(NH)), by adding the crystalline powder of selenourea into mother liquor or cryo-solution with native crystals, in analogy to the classic procedure of heavy-atom derivatization. Selenourea is able to bind to reactive groups at the surface of macromolecules primarily through hydrogen bonds, where the selenium atom may serve as acceptor and amide groups as donors. Selenourea has different chemical properties than heavy-atom reagents and halide ions and provides a convenient way of phasing crystal structures of macromolecules.

PubMed: 27841370
DOI: 10.1038/srep37123

実験手法

X-RAY DIFFRACTION (1.45 Å)