5T3D

Crystal structure of holo-EntF a nonribosomal peptide synthetase in the thioester-forming conformation

Replaces:  4ZXJ

Summary for 5T3D

• Entry DOI: 10.2210/pdb5t3d/pdb
• Descriptor: Enterobactin synthase component F, 5'-({[(2R,3S)-3-amino-4-hydroxy-2-{[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl]sulfanyl}butyl]sulfonyl}amino)-5'-deoxyadenosine (3 entities in total)
• Functional Keywords: nonribosomal peptide synthetase, nrps, condensation, adenylation, pcp, thioesterase, phosphopantetheine, biosynthetic protein
• Biological source: Escherichia coli (strain K12)
• Total number of polymer chains: 1
• Total formula weight: 143087.04

Authors

Miller, B.R.,Drake, E.J.,Sundlov, J.A.,Gulick, A.M. (deposition date: 2016-08-25, release date: 2016-09-21, Last modification date: 2019-12-25)

• Primary citation: Drake, E.J.,Miller, B.R.,Shi, C.,Tarrasch, J.T.,Sundlov, J.A.,Allen, C.L.,Skiniotis, G.,Aldrich, C.C.,Gulick, A.M.; Structures of two distinct conformations of holo-non-ribosomal peptide synthetases.; Nature, 529:235-238, 2016; PubMed: 26762461; DOI: 10.1038/nature16163

Experimental method

X-RAY DIFFRACTION (2.8 Å)