5T2C の概要
| エントリーDOI | 10.2210/pdb5t2c/pdb |
| 関連するPDBエントリー | 5T2A |
| EMDBエントリー | 8343 8345 |
| 分子名称 | 5S rRNA, 60S ribosomal protein L21, 60S ribosomal protein L22, ... (81 entities in total) |
| 機能のキーワード | ribosome |
| 由来する生物種 | Homo sapiens (Human) 詳細 |
| タンパク質・核酸の鎖数 | 81 |
| 化学式量合計 | 3826425.09 |
| 構造登録者 | |
| 主引用文献 | Zhang, X.,Lai, M.,Chang, W.,Yu, I.,Ding, K.,Mrazek, J.,Ng, H.L.,Yang, O.O.,Maslov, D.A.,Zhou, Z.H. Structures and stabilization of kinetoplastid-specific split rRNAs revealed by comparing leishmanial and human ribosomes. Nat Commun, 7:13223-13223, 2016 Cited by PubMed Abstract: The recent success in ribosome structure determination by cryoEM has opened the door to defining structural differences between ribosomes of pathogenic organisms and humans and to understand ribosome-targeting antibiotics. Here, by direct electron-counting cryoEM, we have determined the structures of the Leishmania donovani and human ribosomes at 2.9 Å and 3.6 Å, respectively. Our structure of the leishmanial ribosome elucidates the organization of the six fragments of its large subunit rRNA (as opposed to a single 28S rRNA in most eukaryotes, including humans) and reveals atomic details of a unique 20 amino acid extension of the uL13 protein that pins down the ends of three of the rRNA fragments. The structure also fashions many large rRNA expansion segments. Direct comparison of our human and leishmanial ribosome structures at the decoding A-site sheds light on how the bacterial ribosome-targeting drug paromomycin selectively inhibits the eukaryotic L. donovani, but not human, ribosome. PubMed: 27752045DOI: 10.1038/ncomms13223 主引用文献が同じPDBエントリー |
| 実験手法 | ELECTRON MICROSCOPY (3.6 Å) |
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