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5T1I

CBX3 chromo shadow domain in complex with histone H3 peptide

5T1I の概要
エントリーDOI10.2210/pdb5t1i/pdb
関連するPDBエントリー5T1G
分子名称Chromobox protein homolog 3, Histone H3.1, UNKNOWN ATOM OR ION, ... (4 entities in total)
機能のキーワードstructural genomics, structural genomics consortium, sgc, transcription
由来する生物種Homo sapiens (Human)
詳細
細胞内の位置Nucleus : Q13185 P68431
タンパク質・核酸の鎖数3
化学式量合計17142.59
構造登録者
Liu, Y.,Tempel, W.,Bountra, C.,Arrowsmith, C.H.,Edwards, A.M.,Min, J.,Structural Genomics Consortium (SGC) (登録日: 2016-08-19, 公開日: 2016-09-14, 最終更新日: 2023-10-04)
主引用文献Liu, Y.,Qin, S.,Lei, M.,Tempel, W.,Zhang, Y.,Loppnau, P.,Li, Y.,Min, J.
Peptide recognition by heterochromatin protein 1 (HP1) chromoshadow domains revisited: Plasticity in the pseudosymmetric histone binding site of human HP1.
J. Biol. Chem., 292:5655-5664, 2017
Cited by
PubMed Abstract: Heterochromatin protein 1 (HP1), a highly conserved non-histone chromosomal protein in eukaryotes, plays important roles in the regulation of gene transcription. Each of the three human homologs of HP1 includes a chromoshadow domain (CSD). The CSD interacts with various proteins bearing the PVL motif but also with a region of histone H3 that bears the similar PVL motif. The latter interaction has not yet been resolved in atomic detail. Here we demonstrate that the CSDs of all three human HP1 homologs have comparable affinities to the PVL motif of histone H3. The HP1 C-terminal extension enhances the affinity, as does the increasing length of the H3 peptide. The crystal structure of the human HP1γ CSD (CSD) in complex with an H3 peptide suggests that recognition of H3 by CSD to some extent resembles CSD-PVL interaction. Nevertheless, the prolyl residue of the PVL motif appears to play a role distinct from that of Pro in the known HP1β CSD-PVL complexes. We consequently generalize the historical CSD-PVL interaction model and expand the search scope for additional CSD binding partners.
PubMed: 28223359
DOI: 10.1074/jbc.M116.768374
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (1.6 Å)
構造検証レポート
Validation report summary of 5t1i
検証レポート(詳細版)ダウンロードをダウンロード

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件を2025-07-16に公開中

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