5T0F

Crystal structure of the Myc3 N-terminal domain [44-242] in complex with JAZ10 CMID domain [16-58] from arabidopsis

Summary for 5T0F

• Entry DOI: 10.2210/pdb5t0f/pdb
• Related: 4RQW 4RRU 4RS9
• Descriptor: Transcription factor MYC3, Protein TIFY 9 (3 entities in total)
• Functional Keywords: transcriptional repression, jasmonate signaling, myc3, jaz10 cmid, alternative splicing, desensitization, transcription
• Biological source: Arabidopsis thaliana (Mouse-ear cress); More
• Total number of polymer chains: 2
• Total formula weight: 26840.65

Authors

Ke, J.,Zhang, F.,Brunzelle, J.S.,He, S.Y.,Xu, H.E.,Melcher, K. (deposition date: 2016-08-16, release date: 2017-01-25, Last modification date: 2023-10-04)

Primary citation

Zhang, F.,Ke, J.,Zhang, L.,Chen, R.,Sugimoto, K.,Howe, G.A.,Xu, H.E.,Zhou, M.,He, S.Y.,Melcher, K.
Structural insights into alternative splicing-mediated desensitization of jasmonate signaling.
Proc. Natl. Acad. Sci. U.S.A., 114:1720-1725, 2017

PubMed Abstract: Jasmonate ZIM-domain (JAZ) transcriptional repressors play a key role in regulating jasmonate (JA) signaling in plants. Below a threshold concentration of jasmonoyl isoleucine (JA-Ile), the active form of JA, the C-terminal Jas motif of JAZ proteins binds MYC transcription factors to repress JA signaling. With increasing JA-Ile concentration, the Jas motif binds to JA-Ile and the COI1 subunit of the SCF E3 ligase, which mediates ubiquitination and proteasomal degradation of JAZ repressors, resulting in derepression of MYC transcription factors. JA signaling subsequently becomes desensitized, in part by feedback induction of JAZ splice variants that lack the C-terminal Jas motif but include an N-terminal cryptic MYC-interaction domain (CMID). The CMID sequence is dissimilar to the Jas motif and is incapable of recruiting SCF, allowing CMID-containing JAZ splice variants to accumulate in the presence of JA and to re-repress MYC transcription factors as an integral part of reestablishing signal homeostasis. The mechanism by which the CMID represses MYC transcription factors remains elusive. Here we describe the crystal structure of the MYC3-CMID complex. In contrast to the Jas motif, which forms a single continuous helix when bound to MYC3, the CMID adopts a loop-helix-loop-helix architecture with modular interactions with both the Jas-binding groove and the backside of the Jas-interaction domain of MYC3. This clamp-like interaction allows the CMID to bind MYC3 tightly and block access of MED25 (a subunit of the Mediator coactivator complex) to the MYC3 transcriptional activation domain, shedding light on the enigmatic mechanism by which JAZ splice variants desensitize JA signaling.

PubMed: 28137867
DOI: 10.1073/pnas.1616938114

Experimental method

X-RAY DIFFRACTION (2.4 Å)