5SWJ

Crystal Structure of ATPase delta1-79 Spa47

5SWJ の概要

• エントリーDOI: 10.2210/pdb5swj/pdb
• 関連するPDBエントリー: 5SWL
• 分子名称: Probable ATP synthase SpaL/MxiB, SULFATE ION (3 entities in total)
• 機能のキーワード: atpase, hydrolase
• 由来する生物種: Shigella flexneri
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 78441.04

構造登録者

Morales, Y.,Johnson, S.J.,Burgess, J.L.,Burgess, R.A.,Dickenson, N.E. (登録日: 2016-08-08, 公開日: 2016-11-02, 最終更新日: 2023-10-04)

主引用文献

Burgess, J.L.,Burgess, R.A.,Morales, Y.,Bouvang, J.M.,Johnson, S.J.,Dickenson, N.E.
Structural and Biochemical Characterization of Spa47 Provides Mechanistic Insight into Type III Secretion System ATPase Activation and Shigella Virulence Regulation.
J. Biol. Chem., 291:25837-25852, 2016

PubMed Abstract: Like many Gram-negative pathogens, Shigella rely on a complex type III secretion system (T3SS) to inject effector proteins into host cells, take over host functions, and ultimately establish infection. Despite these critical roles, the energetics and regulatory mechanisms controlling the T3SS and pathogen virulence remain largely unclear. In this study, we present a series of high resolution crystal structures of Spa47 and use the structures to model an activated Spa47 oligomer, finding that ATP hydrolysis may be supported by specific side chain contributions from adjacent protomers within the complex. Follow-up mutagenesis experiments targeting the predicted active site residues validate the oligomeric model and determined that each of the tested residues are essential for Spa47 ATPase activity, although they are not directly responsible for stable oligomer formation. Although N-terminal domain truncation was necessary for crystal formation, it resulted in strictly monomeric Spa47 that is unable to hydrolyze ATP, despite maintaining the canonical ATPase core structure and active site residues. Coupled with studies of ATPase inactive full-length Spa47 point mutants, we find that Spa47 oligomerization and ATP hydrolysis are needed for complete T3SS apparatus formation, a proper translocator secretion profile, and Shigella virulence. This work represents the first structure-function characterization of Spa47, uniquely complementing the multitude of included Shigella T3SS phenotype assays and providing a more complete understanding of T3SS ATPase-mediated pathogen virulence. Additionally, these findings provide a strong platform for follow-up studies evaluating regulation of Spa47 oligomerization in vivo as a much needed means of treating and perhaps preventing shigellosis.

PubMed: 27770024
DOI: 10.1074/jbc.M116.755256

実験手法

X-RAY DIFFRACTION (2.401 Å)