5RYI

INPP5D PanDDA analysis group deposition -- Crystal Structure of the phosphatase and C2 domains of SHIP1 in complex with Z274555794

Summary for 5RYI

• Entry DOI: 10.2210/pdb5ryi/pdb
• Group deposition: INPP5D PanDDA analysis group deposition (G_1002180)
• Descriptor: Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1, N-(3-acetylphenyl)morpholine-4-carboxamide, DIMETHYL SULFOXIDE, ... (4 entities in total)
• Functional Keywords: sgc - diamond i04-1 fragment screening, pandda, xchemexplorer, ship1, alzheimers, hydrolase
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 53360.25

Authors

Bradshaw, W.J.,Newman, J.A.,von Delft, F.,Arrowsmith, C.H.,Edwards, A.M.,Bountra, C.,Gileadi, O. (deposition date: 2020-10-30, release date: 2020-11-11, Last modification date: 2024-02-14)

Primary citation

Bradshaw, W.J.,Kennedy, E.C.,Moreira, T.,Smith, L.A.,Chalk, R.,Katis, V.L.,Benesch, J.L.P.,Brennan, P.E.,Murphy, E.J.,Gileadi, O.
Regulation of inositol 5-phosphatase activity by the C2 domain of SHIP1 and SHIP2.
Structure, 2024

PubMed Abstract: SHIP1, an inositol 5-phosphatase, plays a central role in cellular signaling. As such, it has been implicated in many conditions. Exploiting SHIP1 as a drug target will require structural knowledge and the design of selective small molecules. We have determined apo, and magnesium and phosphate-bound structures of the phosphatase and C2 domains of SHIP1. The C2 domains of SHIP1 and the related SHIP2 modulate the activity of the phosphatase domain. To understand the mechanism, we performed activity assays, hydrogen-deuterium exchange mass spectrometry, and molecular dynamics on SHIP1 and SHIP2. Our findings demonstrate that the influence of the C2 domain is more pronounced for SHIP2 than SHIP1. We determined 91 structures of SHIP1 with fragments bound, with some near the interface between the two domains. We performed a mass spectrometry screen and determined four structures with covalent fragments. These structures could act as starting points for the development of potent, selective probes.

PubMed: 38309262
DOI: 10.1016/j.str.2024.01.005

Experimental method

X-RAY DIFFRACTION (1.45 Å)