5P9M

BTK1 BINDS COVALENTLY TO HY-15771 ONO-4059

5P9M の概要

• エントリーDOI: 10.2210/pdb5p9m/pdb
• Group deposition: Crystal structures of Tyrosine-protein kinase BTK in complex with inhibitors (G_1002011)
• 分子名称: Tyrosine-protein kinase BTK, 6-azanyl-9-[(3~{R})-1-[(~{E})-but-2-enoyl]pyrrolidin-3-yl]-7-(4-phenoxyphenyl)purin-8-one, DIMETHYL SULFOXIDE, ... (4 entities in total)
• 機能のキーワード: kinase, kinase-ligand complex, transferase
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Cytoplasm: Q06187
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32860.70

構造登録者

Gardberg, A.S. (登録日: 2016-09-20, 公開日: 2017-05-24, 最終更新日: 2024-10-09)

主引用文献

Bender, A.T.,Gardberg, A.,Pereira, A.,Johnson, T.,Wu, Y.,Grenningloh, R.,Head, J.,Morandi, F.,Haselmayer, P.,Liu-Bujalski, L.
Ability of Bruton's Tyrosine Kinase Inhibitors to Sequester Y551 and Prevent Phosphorylation Determines Potency for Inhibition of Fc Receptor but not B-Cell Receptor Signaling.
Mol. Pharmacol., 91:208-219, 2017

PubMed Abstract: Bruton's tyrosine kinase (Btk) is expressed in a variety of hematopoietic cells. Btk has been demonstrated to regulate signaling downstream of the B-cell receptor (BCR), Fc receptors (FcRs), and toll-like receptors. It has become an attractive drug target because its inhibition may provide significant efficacy by simultaneously blocking multiple disease mechanisms. Consequently, a large number of Btk inhibitors have been developed. These compounds have diverse binding modes, and both reversible and irreversible inhibitors have been developed. Reported herein, we have tested nine Btk inhibitors and characterized on a molecular level how their interactions with Btk define their ability to block different signaling pathways. By solving the crystal structures of Btk inhibitors bound to the enzyme, we discovered that the compounds can be classified by their ability to trigger sequestration of Btk residue Y551. In cells, we found that sequestration of Y551 renders it inaccessible for phosphorylation. The ability to sequester Y551 was an important determinant of potency against FcεR signaling as Y551 sequestering compounds were more potent for inhibiting basophils and mast cells. This result was true for the inhibition of FcγR signaling as well. In contrast, Y551 sequestration was less a factor in determining potency against BCR signaling. We also found that Btk activity is regulated differentially in basophils and B cells. These results elucidate important determinants for Btk inhibitor potency against different signaling pathways and provide insight for designing new compounds with a broader inhibitory profile that will likely result in greater efficacy.

PubMed: 28062735
DOI: 10.1124/mol.116.107037

実験手法

X-RAY DIFFRACTION (1.41 Å)