5OYL

VSV G CR2

5OYL の概要

• エントリーDOI: 10.2210/pdb5oyl/pdb
• 関連するPDBエントリー: 5OY9
• 分子名称: Glycoprotein, Low-density lipoprotein receptor, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: c, viral protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 52339.61

構造登録者

Albertini, A.A.,Belot, L.,Legrand, P.,Gaudin, Y. (登録日: 2017-09-11, 公開日: 2018-03-21, 最終更新日: 2024-01-17)

主引用文献

Nikolic, J.,Belot, L.,Raux, H.,Legrand, P.,Gaudin, Y.,A Albertini, A.
Structural basis for the recognition of LDL-receptor family members by VSV glycoprotein.
Nat Commun, 9:1029-1029, 2018

PubMed Abstract: Vesicular stomatitis virus (VSV) is an oncolytic rhabdovirus and its glycoprotein G is widely used to pseudotype other viruses for gene therapy. Low-density lipoprotein receptor (LDL-R) serves as a major entry receptor for VSV. Here we report two crystal structures of VSV G in complex with two distinct cysteine-rich domains (CR2 and CR3) of LDL-R, showing that their binding sites on G are identical. We identify two basic residues on G, which are essential for its interaction with CR2 and CR3. Mutating these residues abolishes VSV infectivity even though VSV can use alternative receptors, indicating that all VSV receptors are members of the LDL-R family. Collectively, our data suggest that VSV G has specifically evolved to interact with receptor CR domains. These structural insights into the interaction between VSV G and host cell receptors provide a basis for the design of recombinant viruses with an altered tropism.

PubMed: 29531262
DOI: 10.1038/s41467-018-03432-4

実験手法

X-RAY DIFFRACTION (2.25 Å)