5OXW

Structure of NeqN from Nanoarchaeum equitans

5OXW の概要

• エントリーDOI: 10.2210/pdb5oxw/pdb
• 分子名称: NEQ068, ALA-SER-GLY-SER-PHE-LYS-VAL-ILE-TYR-GLY-ASP (3 entities in total)
• 機能のキーワード: intein complex, splicing
• 由来する生物種: Nanoarchaeum equitans (strain Kin4-M); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 50548.79

構造登録者

Aparicio, D.,Perez-Luque, R.,Ribo, M.,Fita, I. (登録日: 2017-09-07, 公開日: 2018-10-10, 最終更新日: 2024-05-08)

主引用文献

Gordo, V.,Aparicio, D.,Perez-Luque, R.,Benito, A.,Vilanova, M.,Uson, I.,Fita, I.,Ribo, M.
Structural Insights into Subunits Assembly and the Oxyester Splicing Mechanism of Neq pol Split Intein.
Cell Chem Biol, 25:871-879.e2, 2018

PubMed Abstract: Split inteins are expressed as two separated subunits (N-intein and C-intein) fused to the corresponding exteins. The specific association of both intein subunits precedes protein splicing, which results in excision of the intein subunits and in ligation, by a peptide bond, of the concomitant exteins. Catalytically active intein precursors are typically too reactive for crystallization or even isolation. Neq pol is the trans-intein of the B-type DNA polymerase I split gene from hyperthermophile Nanoarchaeum equitans. We have determined the crystal structures of both the isolated NeqN and the complex of NeqN and NeqC subunits carrying the wild-type sequences, including the essential catalytic residues Ser1 and Thr+1, in addition to seven and three residues of the N- and C-exteins, respectively. These structures provide detailed information on the unique oxyester chemistry of the splicing mechanism of Neq pol and of the extensive rearrangements that occur in NeqN during the association step.

PubMed: 29754955
DOI: 10.1016/j.chembiol.2018.04.008

実験手法

X-RAY DIFFRACTION (2.61 Å)