5OQS
Solution structure of antifungal protein NFAP
Summary for 5OQS
| Entry DOI | 10.2210/pdb5oqs/pdb |
| NMR Information | BMRB: 34170 |
| Descriptor | NFAP (1 entity in total) |
| Functional Keywords | structure from cyana 2.1, solution structure, antifungal protein, nfap |
| Biological source | Aspergillus fischeri NRRL 181 |
| Total number of polymer chains | 1 |
| Total formula weight | 6639.57 |
| Authors | Hajdu, D.,Czajlik, A.,Marx, F.,Galgoczy, L.,Batta, G. (deposition date: 2017-08-14, release date: 2018-07-25, Last modification date: 2024-10-23) |
| Primary citation | Hajdu, D.,Huber, A.,Czajlik, A.,Toth, L.,Kele, Z.,Kocsube, S.,Fizil, A.,Marx, F.,Galgoczy, L.,Batta, G. Solution structure and novel insights into phylogeny and mode of action of the Neosartorya (Aspergillus) fischeri antifungal protein (NFAP). Int.J.Biol.Macromol., 129:511-522, 2019 Cited by PubMed Abstract: Small, cysteine-rich and cationic antifungal proteins from natural sources are promising candidates for the development of novel treatment strategies to prevent and combat infections caused by drug-resistant fungi. However, limited information about their structure and antifungal mechanism hampers their future applications. In the present study, we determined the solution structure, dynamics and associated solvent areas of the Neosartorya (Aspergillus) fischeri antifungal protein NFAP. Genome mining within the genus revealed the presence of orthologous genes in N. fischeri and Neosartorya spathulata, and genes encoding closely related proteins can be found in Penicillium brasiliensis and Penicillium oxalicum. We show that the tertiary structure of these putative proteins can be resolved using the structure of NFAP as reliable template for in silico prediction. Localization studies with fluorescence-labelled protein pointed at an energy-dependent uptake mechanism of NFAP in the sensitive model fungus Neurospora crassa and subsequent cytoplasmic localization coincided with cell-death induction. The presented results contribute to a better understanding of the structure/function relationship of NFAP and related proteins and pave the way towards future antifungal drug development. PubMed: 30738898DOI: 10.1016/j.ijbiomac.2019.02.016 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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