5OQS

Solution structure of antifungal protein NFAP

5OQS の概要

• エントリーDOI: 10.2210/pdb5oqs/pdb
• NMR情報: BMRB: 34170
• 分子名称: NFAP (1 entity in total)
• 機能のキーワード: structure from cyana 2.1, solution structure, antifungal protein, nfap
• 由来する生物種: Aspergillus fischeri NRRL 181
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6639.57

構造登録者

Hajdu, D.,Czajlik, A.,Marx, F.,Galgoczy, L.,Batta, G. (登録日: 2017-08-14, 公開日: 2018-07-25, 最終更新日: 2024-10-23)

主引用文献

Hajdu, D.,Huber, A.,Czajlik, A.,Toth, L.,Kele, Z.,Kocsube, S.,Fizil, A.,Marx, F.,Galgoczy, L.,Batta, G.
Solution structure and novel insights into phylogeny and mode of action of the Neosartorya (Aspergillus) fischeri antifungal protein (NFAP).
Int.J.Biol.Macromol., 129:511-522, 2019

PubMed Abstract: Small, cysteine-rich and cationic antifungal proteins from natural sources are promising candidates for the development of novel treatment strategies to prevent and combat infections caused by drug-resistant fungi. However, limited information about their structure and antifungal mechanism hampers their future applications. In the present study, we determined the solution structure, dynamics and associated solvent areas of the Neosartorya (Aspergillus) fischeri antifungal protein NFAP. Genome mining within the genus revealed the presence of orthologous genes in N. fischeri and Neosartorya spathulata, and genes encoding closely related proteins can be found in Penicillium brasiliensis and Penicillium oxalicum. We show that the tertiary structure of these putative proteins can be resolved using the structure of NFAP as reliable template for in silico prediction. Localization studies with fluorescence-labelled protein pointed at an energy-dependent uptake mechanism of NFAP in the sensitive model fungus Neurospora crassa and subsequent cytoplasmic localization coincided with cell-death induction. The presented results contribute to a better understanding of the structure/function relationship of NFAP and related proteins and pave the way towards future antifungal drug development.

PubMed: 30738898
DOI: 10.1016/j.ijbiomac.2019.02.016

実験手法

SOLUTION NMR