5OQN

Crystal structure of the S. cerevisiae condensin Ycg1-Brn1 subcomplex bound to DNA (short kleisin loop)

5OQN の概要

• エントリーDOI: 10.2210/pdb5oqn/pdb
• 分子名称: Condensin complex subunit 3, Condensin complex subunit 2, DNA (5'-D(*GP*AP*TP*GP*TP*GP*TP*AP*GP*CP*TP*AP*CP*AP*CP*AP*TP*C)-3') (3 entities in total)
• 機能のキーワード: kleisin, heat repeat, dna-binding, smc complex, cell cycle
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); 詳細
• 細胞内の位置: Nucleus: Q06680 P38170
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 125871.52

構造登録者

Kschonsak, M.,Hassler, M.,Haering, C.H. (登録日: 2017-08-14, 公開日: 2017-10-18, 最終更新日: 2024-01-17)

主引用文献

Kschonsak, M.,Merkel, F.,Bisht, S.,Metz, J.,Rybin, V.,Hassler, M.,Haering, C.H.
Structural Basis for a Safety-Belt Mechanism That Anchors Condensin to Chromosomes.
Cell, 171:588-600.e24, 2017

PubMed Abstract: Condensin protein complexes coordinate the formation of mitotic chromosomes and thereby ensure the successful segregation of replicated genomes. Insights into how condensin complexes bind to chromosomes and alter their topology are essential for understanding the molecular principles behind the large-scale chromatin rearrangements that take place during cell divisions. Here, we identify a direct DNA-binding site in the eukaryotic condensin complex, which is formed by its Ycg1 HEAT-repeat and Brn1 kleisin subunits. DNA co-crystal structures reveal a conserved, positively charged groove that accommodates the DNA double helix. A peptide loop of the kleisin subunit encircles the bound DNA and, like a safety belt, prevents its dissociation. Firm closure of the kleisin loop around DNA is essential for the association of condensin complexes with chromosomes and their DNA-stimulated ATPase activity. Our data suggest a sophisticated molecular basis for anchoring condensin complexes to chromosomes that enables the formation of large-sized chromatin loops.

PubMed: 28988770
DOI: 10.1016/j.cell.2017.09.008

実験手法

X-RAY DIFFRACTION (3.15 Å)