5OON

Structure of Undecaprenyl-Pyrophosphate Phosphatase, BacA

5OON の概要

• エントリーDOI: 10.2210/pdb5oon/pdb
• 分子名称: Undecaprenyl-diphosphatase, MERCURY (II) ION, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, ... (5 entities in total)
• 機能のキーワード: antibiotic, bacterial cell wall, enzyme mechanism, escherichia coli, in meso in situ serial crystallography, imisx, interdigitated inverted topology repeat, lipid cubic phase, membrane protein, peptidoglycan, phosphatase, pyrophosphorylase, undecaprenyl-pyrophosphate
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32124.28

構造登録者

Huang, C.-Y.,Olieric, V.,Warshamanage, R.,Wang, M.,Howe, N.,Ghachi, M.E.I.,Weichert, D.,Kerff, F.,Stansfeld, P.,Touze, T.,Caffrey, M. (登録日: 2017-08-08, 公開日: 2018-03-21, 最終更新日: 2024-05-08)

主引用文献

El Ghachi, M.,Howe, N.,Huang, C.Y.,Olieric, V.,Warshamanage, R.,Touze, T.,Weichert, D.,Stansfeld, P.J.,Wang, M.,Kerff, F.,Caffrey, M.
Crystal structure of undecaprenyl-pyrophosphate phosphatase and its role in peptidoglycan biosynthesis.
Nat Commun, 9:1078-1078, 2018

PubMed Abstract: As a protective envelope surrounding the bacterial cell, the peptidoglycan sacculus is a site of vulnerability and an antibiotic target. Peptidoglycan components, assembled in the cytoplasm, are shuttled across the membrane in a cycle that uses undecaprenyl-phosphate. A product of peptidoglycan synthesis, undecaprenyl-pyrophosphate, is converted to undecaprenyl-phosphate for reuse in the cycle by the membrane integral pyrophosphatase, BacA. To understand how BacA functions, we determine its crystal structure at 2.6 Å resolution. The enzyme is open to the periplasm and to the periplasmic leaflet via a pocket that extends into the membrane. Conserved residues map to the pocket where pyrophosphorolysis occurs. BacA incorporates an interdigitated inverted topology repeat, a topology type thus far only reported in transporters and channels. This unique topology raises issues regarding the ancestry of BacA, the possibility that BacA has alternate active sites on either side of the membrane and its possible function as a flippase.

PubMed: 29540682
DOI: 10.1038/s41467-018-03477-5

実験手法

X-RAY DIFFRACTION (2.6 Å)