5OND

RfaH from Escherichia coli in complex with ops DNA

5OND の概要

• エントリーDOI: 10.2210/pdb5ond/pdb
• 分子名称: Transcription antitermination protein RfaH, DNA (5'-D(*GP*CP*GP*GP*TP*AP*GP*TP*C)-3') (3 entities in total)
• 機能のキーワード: operon-specific transcription factor, transformer protein, binding of single stranded dna, translation activation, transcription
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 42187.92

構造登録者

Zuber, P.K.,Artsimovitch, I.,Roesch, P.,Knauer, S.H. (登録日: 2017-08-03, 公開日: 2018-06-06, 最終更新日: 2024-01-17)

主引用文献

Zuber, P.K.,Artsimovitch, I.,NandyMazumdar, M.,Liu, Z.,Nedialkov, Y.,Schweimer, K.,Rosch, P.,Knauer, S.H.
The universally-conserved transcription factor RfaH is recruited to a hairpin structure of the non-template DNA strand.
Elife, 7:-, 2018

PubMed Abstract: RfaH, a transcription regulator of the universally conserved NusG/Spt5 family, utilizes a unique mode of recruitment to elongating RNA polymerase to activate virulence genes. RfaH function depends critically on an sequence, an exemplar of a consensus pause, in the non-template DNA strand of the transcription bubble. We used structural and functional analyses to elucidate the role of in RfaH recruitment. Our results demonstrate that induces pausing to facilitate RfaH binding and establishes direct contacts with RfaH. Strikingly, the non-template DNA forms a hairpin in the RfaH: complex structure, flipping out a conserved T residue that is specifically recognized by RfaH. Molecular modeling and genetic evidence support the notion that hairpin is required for RfaH recruitment. We argue that both the sequence and the structure of the non-template strand are read out by transcription factors, expanding the repertoire of transcriptional regulators in all domains of life.

PubMed: 29741479
DOI: 10.7554/eLife.36349

実験手法

X-RAY DIFFRACTION (2.1 Å)