5OMT

Endonuclease NucB

5OMT の概要

• エントリーDOI: 10.2210/pdb5omt/pdb
• 分子名称: NucB (2 entities in total)
• 機能のキーワード: endonuclease, biofilm, bacillus subtilis, dna, hydrolase
• 由来する生物種: Bacillus licheniformis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12014.33

構造登録者

Basle, A.,Lewis, R.J. (登録日: 2017-08-01, 公開日: 2017-11-15, 最終更新日: 2024-11-06)

主引用文献

Basle, A.,Hewitt, L.,Koh, A.,Lamb, H.K.,Thompson, P.,Burgess, J.G.,Hall, M.J.,Hawkins, A.R.,Murray, H.,Lewis, R.J.
Crystal structure of NucB, a biofilm-degrading endonuclease.
Nucleic Acids Res., 46:473-484, 2018

PubMed Abstract: Bacterial biofilms are a complex architecture of cells that grow on moist interfaces, and are held together by a molecular glue of extracellular proteins, sugars and nucleic acids. Biofilms are particularly problematic in human healthcare as they can coat medical implants and are thus a potential source of disease. The enzymatic dispersal of biofilms is increasingly being developed as a new strategy to treat this problem. Here, we have characterized NucB, a biofilm-dispersing nuclease from a marine strain of Bacillus licheniformis, and present its crystal structure together with the biochemistry and a mutational analysis required to confirm its active site. Taken together, these data support the categorization of NucB into a unique subfamily of the ββα metal-dependent non-specific endonucleases. Understanding the structure and function of NucB will facilitate its future development into an anti-biofilm therapeutic agent.

PubMed: 29165717
DOI: 10.1093/nar/gkx1170

実験手法

X-RAY DIFFRACTION (1.35 Å)