5OJY

Co-complex structure of regulator protein 2 (PamR2) with pamamycin 607 from Streptomyces alboniger

5OJY の概要

• エントリーDOI: 10.2210/pdb5ojy/pdb
• 分子名称: TetR family transcription regulator, GLYCEROL, CITRIC ACID, ... (5 entities in total)
• 機能のキーワード: transcription regulator, tetr family transcription regulator, streptomyces alboniger, regulator protein 2, pamamycin biosynthesis, transcription
• 由来する生物種: Streptomyces alboniger
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29266.92

構造登録者

Schmelz, S.,Rebets, Y.,Luzhetskyy, A.,Scrima, A. (登録日: 2017-07-24, 公開日: 2018-04-11, 最終更新日: 2024-11-20)

主引用文献

Rebets, Y.,Schmelz, S.,Gromyko, O.,Tistechok, S.,Petzke, L.,Scrima, A.,Luzhetskyy, A.
Design, development and application of whole-cell based antibiotic-specific biosensor.
Metab. Eng., 47:263-270, 2018

PubMed Abstract: Synthetic biology techniques hold great promise for optimising the production of natural products by microorganisms. However, evaluating the phenotype of a modified bacterium represents a major bottleneck to the engineering cycle - particularly for antibiotic-producing actinobacteria strains, which grow slowly and are challenging to genetically manipulate. Here, we report the generation and application of antibiotic-specific whole-cell biosensor derived from TetR transcriptional repressor for use in identifying and optimising antibiotic producers. The constructed biosensor was successfully used to improve production of polyketide antibiotic pamamycin. However, an initial biosensor based on native genetic elements had inadequate dynamic and operating ranges. To overcome these limitations, we fine-tuned biosensor performance through alterations of the promoter and operator of output module and the ligand affinity of transcription factor module, which enabled us to deduce recommendations for building and application of actinobacterial biosensors.

PubMed: 29609044
DOI: 10.1016/j.ymben.2018.03.019

実験手法

X-RAY DIFFRACTION (1.851 Å)