5OJ3

YCF48 from Cyanidioschyzon merolae

5OJ3 の概要

• エントリーDOI: 10.2210/pdb5oj3/pdb
• 分子名称: Photosystem II stability/assembly factor HCF136 (1 entity in total)
• 機能のキーワード: photosystem ii, photosynthesis
• 由来する生物種: Cyanidioschyzon merolae (strain 10D) (Red alga)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 95124.95

構造登録者

Michoux, F.,Murray, J.W.,Nixon, P.J. (登録日: 2017-07-20, 公開日: 2018-08-08, 最終更新日: 2024-01-17)

主引用文献

Yu, J.,Knoppova, J.,Michoux, F.,Bialek, W.,Cota, E.,Shukla, M.K.,Straskova, A.,Pascual Aznar, G.,Sobotka, R.,Komenda, J.,Murray, J.W.,Nixon, P.J.
Ycf48 involved in the biogenesis of the oxygen-evolving photosystem II complex is a seven-bladed beta-propeller protein.
Proc. Natl. Acad. Sci. U.S.A., 115:E7824-E7833, 2018

PubMed Abstract: Robust photosynthesis in chloroplasts and cyanobacteria requires the participation of accessory proteins to facilitate the assembly and maintenance of the photosynthetic apparatus located within the thylakoid membranes. The highly conserved Ycf48 protein acts early in the biogenesis of the oxygen-evolving photosystem II (PSII) complex by binding to newly synthesized precursor D1 subunit and by promoting efficient association with the D2 protein to form a PSII reaction center (PSII RC) assembly intermediate. Ycf48 is also required for efficient replacement of damaged D1 during the repair of PSII. However, the structural features underpinning Ycf48 function remain unclear. Here we show that Ycf48 proteins encoded by the thermophilic cyanobacterium and the red alga form seven-bladed beta-propellers with the 19-aa insertion characteristic of eukaryotic Ycf48 located at the junction of blades 3 and 4. Knowledge of these structures has allowed us to identify a conserved "Arg patch" on the surface of Ycf48 that is important for binding of Ycf48 to PSII RCs but also to larger complexes, including trimeric photosystem I (PSI). Reduced accumulation of chlorophyll in the absence of Ycf48 and the association of Ycf48 with PSI provide evidence of a more wide-ranging role for Ycf48 in the biogenesis of the photosynthetic apparatus than previously thought. Copurification of Ycf48 with the cyanobacterial YidC protein insertase supports the involvement of Ycf48 during the cotranslational insertion of chlorophyll-binding apopolypeptides into the membrane.

PubMed: 30061392
DOI: 10.1073/pnas.1800609115

実験手法

X-RAY DIFFRACTION (2.982 Å)