5OF2

The structural versatility of TasA in B. subtilis biofilm formation

5OF2 の概要

• エントリーDOI: 10.2210/pdb5of2/pdb
• 分子名称: Spore coat-associated protein N, 1,2-ETHANEDIOL, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: tasa, biofilm matrix, amyloid fiber, xray structure, protein fibril
• 由来する生物種: Bacillus subtilis (strain 168)
• 細胞内の位置: Secreted : P54507
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23254.96

構造登録者

Roske, Y.,Diehl, A.,Ball, L.,Chowdhury, A.,Hiller, M.,Moliere, N.,Kramer, R.,Nagaraj, M.,Stoeppler, D.,Worth, C.L.,Schlegel, B.,Leidert, M.,Cremer, N.,Eisenmenger, F.,Lopez, D.,Schmieder, P.,Heinemann, U.,Turgay, K.,Akbey, U.,Oschkinat, H. (登録日: 2017-07-10, 公開日: 2018-03-21, 最終更新日: 2024-01-17)

主引用文献

Diehl, A.,Roske, Y.,Ball, L.,Chowdhury, A.,Hiller, M.,Moliere, N.,Kramer, R.,Stoppler, D.,Worth, C.L.,Schlegel, B.,Leidert, M.,Cremer, N.,Erdmann, N.,Lopez, D.,Stephanowitz, H.,Krause, E.,van Rossum, B.J.,Schmieder, P.,Heinemann, U.,Turgay, K.,Akbey, U.,Oschkinat, H.
Structural changes of TasA in biofilm formation ofBacillus subtilis.
Proc. Natl. Acad. Sci. U.S.A., 115:3237-3242, 2018

PubMed Abstract: Microorganisms form surface-attached communities, termed biofilms, which can serve as protection against host immune reactions or antibiotics. biofilms contain TasA as major proteinaceous component in addition to exopolysaccharides. In stark contrast to the initially unfolded biofilm proteins of other bacteria, TasA is a soluble, stably folded monomer, whose structure we have determined by X-ray crystallography. Subsequently, we characterized in vitro different oligomeric forms of TasA by NMR, EM, X-ray diffraction, and analytical ultracentrifugation (AUC) experiments. However, by magic-angle spinning (MAS) NMR on live biofilms, a swift structural change toward only one of these forms, consisting of homogeneous and protease-resistant, β-sheet-rich fibrils, was observed in vivo. Thereby, we characterize a structural change from a globular state to a fibrillar form in a functional prokaryotic system on the molecular level.

PubMed: 29531041
DOI: 10.1073/pnas.1718102115

実験手法

X-RAY DIFFRACTION (1.86 Å)