5OEM

Crystal Structure of Interferon Regulatory Factor 9 IAD Domain

5OEM の概要

• エントリーDOI: 10.2210/pdb5oem/pdb
• 分子名称: Interferon regulatory factor 9, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: irf9, transcription
• 由来する生物種: Mus musculus (Mouse)
• 細胞内の位置: Nucleus: Q61179
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21337.95

構造登録者

Rengachari, S.,Panne, D. (登録日: 2017-07-09, 公開日: 2018-01-24, 最終更新日: 2024-01-17)

主引用文献

Rengachari, S.,Groiss, S.,Devos, J.M.,Caron, E.,Grandvaux, N.,Panne, D.
Structural basis of STAT2 recognition by IRF9 reveals molecular insights into ISGF3 function.
Proc. Natl. Acad. Sci. U.S.A., 115:E601-E609, 2018

PubMed Abstract: Cytokine signaling through the JAK/STAT pathway controls multiple cellular responses including growth, survival, differentiation, and pathogen resistance. An expansion in the gene regulatory repertoire controlled by JAK/STAT signaling occurs through the interaction of STATs with IRF transcription factors to form ISGF3, a complex that contains STAT1, STAT2, and IRF9 and regulates expression of IFN-stimulated genes. ISGF3 function depends on selective interaction between IRF9, through its IRF-association domain (IAD), with the coiled-coil domain (CCD) of STAT2. Here, we report the crystal structures of the IRF9-IAD alone and in a complex with STAT2-CCD. Despite similarity in the overall structure among respective paralogs, the surface features of the IRF9-IAD and STAT2-CCD have diverged to enable specific interaction between these family members. We derive a model for the ISGF3 complex bound to an ISRE DNA element and demonstrate that the observed interface between STAT2 and IRF9 is required for ISGF3 function in cells.

PubMed: 29317535
DOI: 10.1073/pnas.1718426115

実験手法

X-RAY DIFFRACTION (1.9 Å)