5OAC

FLiP major capsid protein

5OAC の概要

• エントリーDOI: 10.2210/pdb5oac/pdb
• EMDBエントリー: 3771
• 分子名称: Major capsid protein (1 entity in total)
• 機能のキーワード: capsid, major capsid protein, virion, virus
• 由来する生物種: unidentified phage
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 345339.34

構造登録者

De Colibus, L.,Stuart, D.I.,Huiskonen, J.T. (登録日: 2017-06-21, 公開日: 2017-07-26, 最終更新日: 2024-11-06)

主引用文献

Laanto, E.,Mantynen, S.,De Colibus, L.,Marjakangas, J.,Gillum, A.,Stuart, D.I.,Ravantti, J.J.,Huiskonen, J.T.,Sundberg, L.R.
Virus found in a boreal lake links ssDNA and dsDNA viruses.
Proc. Natl. Acad. Sci. U.S.A., 114:8378-8383, 2017

PubMed Abstract: Viruses have impacted the biosphere in numerous ways since the dawn of life. However, the evolution, genetic, structural, and taxonomic diversity of viruses remain poorly understood, in part because sparse sampling of the virosphere has concentrated mostly on exploring the abundance and diversity of dsDNA viruses. Furthermore, viral genomes are highly diverse, and using only the current sequence-based methods for classifying viruses and studying their phylogeny is complicated. Here we describe a virus, FLiP (-infecting, lipid-containing phage), with a circular ssDNA genome and an internal lipid membrane enclosed in the icosahedral capsid. The 9,174-nt-long genome showed limited sequence similarity to other known viruses. The genetic data imply that this virus might use replication mechanisms similar to those found in other ssDNA replicons. However, the structure of the viral major capsid protein, elucidated at near-atomic resolution using cryo-electron microscopy, is strikingly similar to that observed in dsDNA viruses of the PRD1-adenovirus lineage, characterized by a major capsid protein bearing two β-barrels. The strong similarity between FLiP and another member of the structural lineage, bacteriophage PM2, extends to the capsid organization (pseudo = 21 ) despite the difference in the genetic material packaged and the lack of significant sequence similarity.

PubMed: 28716906
DOI: 10.1073/pnas.1703834114

実験手法

ELECTRON MICROSCOPY (4 Å)