5O8W

CRYSTAL STRUCTURE ANALYSIS OF THE YEAST ELONGATION FACTOR COMPLEX EEF1A:EEF1BA

5O8W の概要

• エントリーDOI: 10.2210/pdb5o8w/pdb
• 分子名称: Elongation factor 1-alpha, Elongation factor 1-beta, GLUTAMINE, ... (5 entities in total)
• 機能のキーワード: protein-protein complex, translation, covalent modification
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 62056.74

構造登録者

Wirth, C.,Andersen, G.R.,Hunte, C. (登録日: 2017-06-14, 公開日: 2017-08-23, 最終更新日: 2025-04-09)

主引用文献

Jank, T.,Belyi, Y.,Wirth, C.,Rospert, S.,Hu, Z.,Dengjel, J.,Tzivelekidis, T.,Andersen, G.R.,Hunte, C.,Schlosser, A.,Aktories, K.
Protein glutaminylation is a yeast-specific posttranslational modification of elongation factor 1A.
J. Biol. Chem., 292:16014-16023, 2017

PubMed Abstract: Ribosomal translation factors are fundamental for protein synthesis and highly conserved in all kingdoms of life. The essential eukaryotic elongation factor 1A (eEF1A) delivers aminoacyl tRNAs to the A-site of the translating 80S ribosome. Several studies have revealed that eEF1A is posttranslationally modified. Using MS analysis, site-directed mutagenesis, and X-ray structural data analysis of eEF1A, we identified a posttranslational modification in which the α amino group of mono-l-glutamine is covalently linked to the side chain of glutamate 45 in eEF1A. The MS analysis suggested that all eEF1A molecules are modified by this glutaminylation and that this posttranslational modification occurs at all stages of yeast growth. The mutational studies revealed that this glutaminylation is not essential for the normal functions of eEF1A in However, eEF1A glutaminylation slightly reduced growth under antibiotic-induced translational stress conditions. Moreover, we identified the same posttranslational modification in eEF1A from but not in various other eukaryotic organisms tested despite strict conservation of the Glu residue among these organisms. We therefore conclude that eEF1A glutaminylation is a yeast-specific posttranslational modification that appears to influence protein translation.

PubMed: 28801462
DOI: 10.1074/jbc.M117.801035

実験手法

X-RAY DIFFRACTION (1.67 Å)