5O8O

N. crassa Tom40 model based on cryo-EM structure of the TOM core complex at 6.8 A

5O8O の概要

• エントリーDOI: 10.2210/pdb5o8o/pdb
• EMDBエントリー: 3761
• 分子名称: Mitochondrial import receptor subunit tom40 (1 entity in total)
• 機能のキーワード: tom-complex, protein import, mitochondria, cryo-em, protein transport
• 由来する生物種: Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 38184.80

構造登録者

Bausewein, T.,Mills, D.J.,Nussberger, S.,Nitschke, B.,Kuehlbrandt, W. (登録日: 2017-06-13, 公開日: 2017-08-16, 最終更新日: 2024-05-15)

主引用文献

Bausewein, T.,Mills, D.J.,Langer, J.D.,Nitschke, B.,Nussberger, S.,Kuhlbrandt, W.
Cryo-EM Structure of the TOM Core Complex from Neurospora crassa.
Cell, 170:693-700.e7, 2017

PubMed Abstract: The TOM complex is the main entry gate for protein precursors from the cytosol into mitochondria. We have determined the structure of the TOM core complex by cryoelectron microscopy (cryo-EM). The complex is a 148 kDa symmetrical dimer of ten membrane protein subunits that create a shallow funnel on the cytoplasmic membrane surface. In the core of the dimer, the β-barrels of the Tom40 pore form two identical preprotein conduits. Each Tom40 pore is surrounded by the transmembrane segments of the α-helical subunits Tom5, Tom6, and Tom7. Tom22, the central preprotein receptor, connects the two Tom40 pores at the dimer interface. Our structure offers detailed insights into the molecular architecture of the mitochondrial preprotein import machinery.

PubMed: 28802041
DOI: 10.1016/j.cell.2017.07.012

実験手法

ELECTRON MICROSCOPY (6.8 Å)