5O8B

Difference-refined excited-state structure of rsEGFP2 1ps following 400nm-laser irradiation of the off-state.

Summary for 5O8B

• Entry DOI: 10.2210/pdb5o8b/pdb
• Related: 5dtx 5dty
• Descriptor: Green fluorescent protein (2 entities in total)
• Functional Keywords: photoswitching on state fluorescent state chromophore sfx serial femtosecond crystallography cxi lcls time resolved crystallography chromohore isomerisation, fluorescent protein
• Biological source: Aequorea victoria (Jellyfish)
• Total number of polymer chains: 1
• Total formula weight: 28099.69

Authors

Coquelle, N.,Sliwa, M.,Woodhouse, J.,Schiro, G.,Adam, V.,Aquila, A.,Barends, T.R.M.,Boutet, S.,Byrdin, M.,Carbajo, S.,De la Mora, E.,Doak, R.B.,Feliks, M.,Fieschi, F.,Foucar, L.,Guillon, V.,Hilpert, M.,Hunter, M.,Jakobs, S.,Koglin, J.E.,Kovacsova, G.,Lane, T.J.,Levy, B.,Liang, M.,Nass, K.,Ridard, J.,Robinson, J.S.,Roome, C.M.,Ruckebusch, C.,Seaberg, M.,Thepaut, M.,Cammarata, M.,Demachy, I.,Field, M.,Shoeman, R.L.,Bourgeois, D.,Colletier, J.P.,Schlichting, I.,Weik, M. (deposition date: 2017-06-12, release date: 2018-01-24, Last modification date: 2024-01-17)

• Primary citation: Coquelle, N.,Sliwa, M.,Woodhouse, J.,Schiro, G.,Adam, V.,Aquila, A.,Barends, T.R.M.,Boutet, S.,Byrdin, M.,Carbajo, S.,De la Mora, E.,Doak, R.B.,Feliks, M.,Fieschi, F.,Foucar, L.,Guillon, V.,Hilpert, M.,Hunter, M.S.,Jakobs, S.,Koglin, J.E.,Kovacsova, G.,Lane, T.J.,Levy, B.,Liang, M.,Nass, K.,Ridard, J.,Robinson, J.S.,Roome, C.M.,Ruckebusch, C.,Seaberg, M.,Thepaut, M.,Cammarata, M.,Demachy, I.,Field, M.,Shoeman, R.L.,Bourgeois, D.,Colletier, J.P.,Schlichting, I.,Weik, M.; Chromophore twisting in the excited state of a photoswitchable fluorescent protein captured by time-resolved serial femtosecond crystallography.; Nat Chem, 10:31-37, 2018; PubMed: 29256511; DOI: 10.1038/nchem.2853

Experimental method

X-RAY DIFFRACTION (1.7 Å)