Crystal Structure of rsEGFP2 in the fluorescent on-state determined by SFX

Summary for 5O89

Related5DTX 5DTY
DescriptorGreen fluorescent protein (2 entities in total)
Functional Keywordsphotoswitching on state fluorescent state chromophore sfx serial femtosecond crystallography cxi lcls time resolved crystallography chromohore isomerisation, fluorescent protein
Biological sourceAequorea victoria (Jellyfish)
Total number of polymer chains1
Total molecular weight28099.69
Primary citation
Coquelle, N.,Sliwa, M.,Woodhouse, J.,Schiro, G.,Adam, V.,Aquila, A.,Barends, T.R.M.,Boutet, S.,Byrdin, M.,Carbajo, S.,De la Mora, E.,Doak, R.B.,Feliks, M.,Fieschi, F.,Foucar, L.,Guillon, V.,Hilpert, M.,Hunter, M.S.,Jakobs, S.,Koglin, J.E.,Kovacsova, G.,Lane, T.J.,Levy, B.,Liang, M.,Nass, K.,Ridard, J.,Robinson, J.S.,Roome, C.M.,Ruckebusch, C.,Seaberg, M.,Thepaut, M.,Cammarata, M.,Demachy, I.,Field, M.,Shoeman, R.L.,Bourgeois, D.,Colletier, J.P.,Schlichting, I.,Weik, M.
Chromophore twisting in the excited state of a photoswitchable fluorescent protein captured by time-resolved serial femtosecond crystallography.
Nat Chem, 10:31-37, 2018
PubMed: 29256511 (PDB entries with the same primary citation)
DOI: 10.1038/nchem.2853
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.174400 6.6%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation report