5O89

Crystal Structure of rsEGFP2 in the fluorescent on-state determined by SFX

5O89 の概要

• エントリーDOI: 10.2210/pdb5o89/pdb
• 関連するPDBエントリー: 5DTX 5DTY
• 分子名称: Green fluorescent protein (2 entities in total)
• 機能のキーワード: photoswitching on state fluorescent state chromophore sfx serial femtosecond crystallography cxi lcls time resolved crystallography chromohore isomerisation, fluorescent protein
• 由来する生物種: Aequorea victoria (Jellyfish)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28099.69

構造登録者

Coquelle, N.,Sliwa, M.,Woodhouse, J.,Schiro, G.,Adam, V.,Aquila, A.,Barends, T.R.M.,Boutet, S.,Byrdin, M.,Carbajo, S.,De la Mora, E.,Doak, R.B.,Feliks, M.,Fieschi, F.,Foucar, L.,Guillon, V.,Hilpert, M.,Hunter, M.,Jakobs, S.,Koglin, J.E.,Kovacsova, G.,Lane, T.J.,Levy, B.,Liang, M.,Nass, K.,Ridard, J.,Robinson, J.S.,Roome, C.M.,Ruckebusch, C.,Seaberg, M.,Thepaut, M.,Cammarata, M.,Demachy, I.,Field, M.,Shoeman, R.L.,Bourgeois, D.,Colletier, J.P.,Schlichting, I.,Weik, M. (登録日: 2017-06-12, 公開日: 2017-12-13, 最終更新日: 2024-01-17)

• 主引用文献: Coquelle, N.,Sliwa, M.,Woodhouse, J.,Schiro, G.,Adam, V.,Aquila, A.,Barends, T.R.M.,Boutet, S.,Byrdin, M.,Carbajo, S.,De la Mora, E.,Doak, R.B.,Feliks, M.,Fieschi, F.,Foucar, L.,Guillon, V.,Hilpert, M.,Hunter, M.S.,Jakobs, S.,Koglin, J.E.,Kovacsova, G.,Lane, T.J.,Levy, B.,Liang, M.,Nass, K.,Ridard, J.,Robinson, J.S.,Roome, C.M.,Ruckebusch, C.,Seaberg, M.,Thepaut, M.,Cammarata, M.,Demachy, I.,Field, M.,Shoeman, R.L.,Bourgeois, D.,Colletier, J.P.,Schlichting, I.,Weik, M.; Chromophore twisting in the excited state of a photoswitchable fluorescent protein captured by time-resolved serial femtosecond crystallography.; Nat Chem, 10:31-37, 2018; PubMed: 29256511; DOI: 10.1038/nchem.2853

実験手法

X-RAY DIFFRACTION (1.7 Å)