5O7U

Crystal structure of the 7-Fluorotryptophan RSL lectin in complex with Lewis x tetrasaccharide

5O7U の概要

• エントリーDOI: 10.2210/pdb5o7u/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900119
• 分子名称: Putative fucose-binding lectin protein, alpha-L-fucopyranose-(1-3)-[beta-D-galactopyranose-(1-4)]2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-L-fucopyranose-(1-3)-[beta-D-galactopyranose-(1-4)]2-acetamido-2-deoxy-beta-D-glucopyranose-(1-3)-alpha-D-galactopyranose, ... (8 entities in total)
• 機能のキーワード: lectin, fluorinated tryptophan, lewis x, carbohydrate, sugar binding protein
• 由来する生物種: Ralstonia solanacearum
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 22087.79

構造登録者

Varrot, A. (登録日: 2017-06-09, 公開日: 2018-06-06, 最終更新日: 2024-11-06)

主引用文献

Tobola, F.,Lelimousin, M.,Varrot, A.,Gillon, E.,Darnhofer, B.,Blixt, O.,Birner-Gruenberger, R.,Imberty, A.,Wiltschi, B.
Effect of Noncanonical Amino Acids on Protein-Carbohydrate Interactions: Structure, Dynamics, and Carbohydrate Affinity of a Lectin Engineered with Fluorinated Tryptophan Analogs.
ACS Chem. Biol., 13:2211-2219, 2018

PubMed Abstract: Protein-carbohydrate interactions play crucial roles in biology. Understanding and modifying these interactions is of major interest for fighting many diseases. We took a synthetic biology approach and incorporated noncanonical amino acids into a bacterial lectin to modulate its interactions with carbohydrates. We focused on tryptophan, which is prevalent in carbohydrate binding sites. The exchange of the tryptophan residues with analogs fluorinated at different positions resulted in three distinctly fluorinated variants of the lectin from Ralstonia solanacearum. We observed differences in stability and affinity toward fucosylated glycans and rationalized them by X-ray and modeling studies. While fluorination decreased the aromaticity of the indole ring and, therefore, the strength of carbohydrate-aromatic interactions, additional weak hydrogen bonds were formed between fluorine and the ligand hydroxyl groups. Our approach opens new possibilities to engineer carbohydrate receptors.

PubMed: 29812892
DOI: 10.1021/acschembio.8b00377

実験手法

X-RAY DIFFRACTION (1.15 Å)