5O7J

Structural insights into the periplasmic sensor domain of the GacS histidine kinase controlling biofilm formation in Pseudomonas aeruginosa

5O7J の概要

• エントリーDOI: 10.2210/pdb5o7j/pdb
• NMR情報: BMRB: 26840
• 分子名称: Histidine kinase (1 entity in total)
• 機能のキーワード: periplasmic detection domain, histidine-kinase, gacs, biofilm, signaling protein
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16375.36

構造登録者

Ali-Ahmad, A.,Bornet, O.,Fadel, F.,Bourne, Y.,Vincent, F.,Guerlesquin, F.,Sebban-Kreuzer, C. (登録日: 2017-06-09, 公開日: 2018-02-07, 最終更新日: 2024-06-19)

主引用文献

Ali-Ahmad, A.,Fadel, F.,Sebban-Kreuzer, C.,Ba, M.,Pelissier, G.D.,Bornet, O.,Guerlesquin, F.,Bourne, Y.,Bordi, C.,Vincent, F.
Structural and functional insights into the periplasmic detector domain of the GacS histidine kinase controlling biofilm formation in Pseudomonas aeruginosa.
Sci Rep, 7:11262-11262, 2017

PubMed Abstract: Pseudomonas aeruginosa is an opportunistic pathogenic bacterium responsible for both acute and chronic infections and has developed resistance mechanisms due to its ability to promote biofilm formation and evade host adaptive immune responses. Here, we investigate the functional role of the periplasmic detector domain (GacS) from the membrane-bound GacS histidine kinase, which is one of the key players for biofilm formation and coordination of bacterial lifestyles. A gacS mutant devoid of the periplasmic detector domain is severely defective in biofilm formation. Functional assays indicate that this effect is accompanied by concomitant changes in the expression of the two RsmY/Z small RNAs that control activation of GacA-regulated genes. The solution NMR structure of GacS reveals a distinct PDC/PAS α/β fold characterized by a three-stranded β-sheet flanked by α-helices and an atypical major loop. Point mutations in a putative ligand binding pocket lined by positively-charged residues originating primarily from the major loop impaired biofilm formation. These results demonstrate the functional role of GacS, evidence critical residues involved in GacS/GacA signal transduction system that regulates biofilm formation, and document the evolutionary diversity of the PDC/PAS domain fold in bacteria.

PubMed: 28900144
DOI: 10.1038/s41598-017-11361-3

実験手法

SOLUTION NMR