5O69

The structure of the thermobifida fusca guanidine III riboswitch with agmatine.

5O69 の概要

• エントリーDOI: 10.2210/pdb5o69/pdb
• 分子名称: RNA (41-MER), RNA (37-MER), AGMATINE, ... (5 entities in total)
• 機能のキーワード: guanidine iii riboswitch, stem-loop, pseudoknot, rna, gene regulation
• 由来する生物種: Thermobifida fusca; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 27036.10

構造登録者

Huang, L.,Wang, J.,Lilley, D.M.J. (登録日: 2017-06-06, 公開日: 2017-10-18, 最終更新日: 2024-05-08)

主引用文献

Huang, L.,Wang, J.,Wilson, T.J.,Lilley, D.M.J.
Structure of the Guanidine III Riboswitch.
Cell Chem Biol, 24:1407-1415.e2, 2017

PubMed Abstract: Riboswitches are structural elements found in mRNA molecules that couple small-molecule binding to regulation of gene expression, usually by controlling transcription or translation. We have determined high-resolution crystal structures of the ykkC guanidine III riboswitch from Thermobifida fusca. The riboswitch forms a classic H-type pseudoknot that includes a triple helix that is continuous with a central core of conserved nucleotides. These form a left-handed helical ramp of inter-nucleotide interactions, generating the guanidinium cation binding site. The ligand is hydrogen bonded to the Hoogsteen edges of two guanine bases. The binding pocket has a side opening that can accommodate a small side chain, shown by structures with bound methylguanidine, aminoguanidine, ethylguanidine, and agmatine. Comparison of the new structure with those of the guanidine I and II riboswitches reveals that evolution generated three different structural solutions for guanidine binding and subsequent gene regulation, although with some common elements.

PubMed: 28988949
DOI: 10.1016/j.chembiol.2017.08.021

実験手法

X-RAY DIFFRACTION (2.319 Å)