5O64

From macrocrystals to microcrystals: a strategy for membrane protein serial crystallography

5O64 の概要

• エントリーDOI: 10.2210/pdb5o64/pdb
• 分子名称: Photosynthetic reaction center cytochrome c subunit, HEPTANE-1,2,3-TRIOL, BACTERIOCHLOROPHYLL B, ... (16 entities in total)
• 機能のキーワード: membrane photosynthetic wild-type xfel, membrane protein
• 由来する生物種: Blastochloris viridis; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 144507.53

構造登録者

Dods, R.,Baath, P.,Branden, G.,Neutze, R. (登録日: 2017-06-05, 公開日: 2017-08-16, 最終更新日: 2024-01-31)

主引用文献

Dods, R.,Bath, P.,Arnlund, D.,Beyerlein, K.R.,Nelson, G.,Liang, M.,Harimoorthy, R.,Berntsen, P.,Malmerberg, E.,Johansson, L.,Andersson, R.,Bosman, R.,Carbajo, S.,Claesson, E.,Conrad, C.E.,Dahl, P.,Hammarin, G.,Hunter, M.S.,Li, C.,Lisova, S.,Milathianaki, D.,Robinson, J.,Safari, C.,Sharma, A.,Williams, G.,Wickstrand, C.,Yefanov, O.,Davidsson, J.,DePonte, D.P.,Barty, A.,Branden, G.,Neutze, R.
From Macrocrystals to Microcrystals: A Strategy for Membrane Protein Serial Crystallography.
Structure, 25:1461-1468.e2, 2017

PubMed Abstract: Serial protein crystallography was developed at X-ray free-electron lasers (XFELs) and is now also being applied at storage ring facilities. Robust strategies for the growth and optimization of microcrystals are needed to advance the field. Here we illustrate a generic strategy for recovering high-density homogeneous samples of microcrystals starting from conditions known to yield large (macro) crystals of the photosynthetic reaction center of Blastochloris viridis (RC). We first crushed these crystals prior to multiple rounds of microseeding. Each cycle of microseeding facilitated improvements in the RC serial femtosecond crystallography (SFX) structure from 3.3-Å to 2.4-Å resolution. This approach may allow known crystallization conditions for other proteins to be adapted to exploit novel scientific opportunities created by serial crystallography.

PubMed: 28781082
DOI: 10.1016/j.str.2017.07.002

実験手法

X-RAY DIFFRACTION (3.3 Å)