5O3K

Crystal Structure of mutant M54L/M64L/M96L of Two-Domain Laccase from Streptomyces griseoflavus with 1 mM copper sulfate on growth medium

5O3K の概要

• エントリーDOI: 10.2210/pdb5o3k/pdb
• 分子名称: Two-domain laccase, COPPER (II) ION, OXYGEN ATOM, ... (6 entities in total)
• 機能のキーワード: two-domain laccase, laccase, streptomyces griseoflavus, oxidoreductase
• 由来する生物種: Streptomyces griseoflavus
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 419236.40

構造登録者

Gabdulkhakov, A.G.,Tishchenko, T.V. (登録日: 2017-05-24, 公開日: 2018-03-28, 最終更新日: 2024-01-17)

主引用文献

Gabdulkhakov, A.G.,Kostareva, O.S.,Kolyadenko, I.A.,Mikhaylina, A.O.,Trubitsina, L.I.,Tishchenko, S.V.
Incorporation of Copper Ions into T2/T3 Centers of Two-Domain Laccases.
Mol.Biol.(Moscow), 52:29-35, 2018

PubMed Abstract: Laccase belongs to the family of copper-containing oxidases. A study was made of the mechanism that sustains the incorporation of copper ions into the T2/T3 centers of recombinant two-domain laccase Streptomyces griseoflavus Ac-993. The occupancy of the T3 center by copper ions was found to increase with an increasing copper content in the culture medium and after dialysis of the protein preparation against a copper sulfate-containing buffer. The T2 center was filled only when overproducer strain cells were grown at a higher copper concentration in the medium. Two-domain laccases were assumed to possess a channel that serves to deliver copper ions to the T3 center during the formation of the three-dimensional laccase conformation and dialysis of the protein preparation. A narrower channel leads to the T2 center in two-domain laccases compared with three-domain ones, rendering the center less accessible for copper atoms. The incorporation of copper ions into the T2 center of two-domain laccases is likely to occur in the course of their biosynthesis or the formation of a functional trimer.

PubMed: 29512633
DOI: 10.7868/S0026898418010056

実験手法

X-RAY DIFFRACTION (2.1 Å)