5O09

BtubABC mini microtubule

これはPDB形式変換不可エントリーです。

5O09 の概要

• エントリーDOI: 10.2210/pdb5o09/pdb
• 関連するPDBエントリー: 5O01
• EMDBエントリー: 3726
• 分子名称: Tubulin, Tubulin BtubB, Bacterial kinesin light chain, ... (4 entities in total)
• 機能のキーワード: bacterial cytoskeleton, microtubules, structural protein
• 由来する生物種: Prosthecobacter dejongeii; 詳細
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 972473.56

構造登録者

Deng, X.,Bharat, T.A.M.,Lowe, J. (登録日: 2017-05-16, 公開日: 2017-07-19, 最終更新日: 2024-05-15)

主引用文献

Deng, X.,Fink, G.,Bharat, T.A.M.,He, S.,Kureisaite-Ciziene, D.,Lowe, J.
Four-stranded mini microtubules formed by Prosthecobacter BtubAB show dynamic instability.
Proc. Natl. Acad. Sci. U.S.A., 114:E5950-E5958, 2017

PubMed Abstract: Microtubules, the dynamic, yet stiff hollow tubes built from αβ-tubulin protein heterodimers, are thought to be present only in eukaryotic cells. Here, we report a 3.6-Å helical reconstruction electron cryomicroscopy structure of four-stranded mini microtubules formed by bacterial tubulin-like BtubAB proteins. Despite their much smaller diameter, mini microtubules share many key structural features with eukaryotic microtubules, such as an M-loop, alternating subunits, and a seam that breaks overall helical symmetry. Using in vitro total internal reflection fluorescence microscopy, we show that bacterial mini microtubules treadmill and display dynamic instability, another hallmark of eukaryotic microtubules. The third protein in the gene cluster, BtubC, previously known as "bacterial kinesin light chain," binds along protofilaments every 8 nm, inhibits BtubAB mini microtubule catastrophe, and increases rescue. Our work reveals that some bacteria contain regulated and dynamic cytomotive microtubule systems that were once thought to be only useful in much larger and sophisticated eukaryotic cells.

PubMed: 28673988
DOI: 10.1073/pnas.1705062114

実験手法

ELECTRON MICROSCOPY (3.6 Å)