5NTB

Streptomyces papain inhibitor (SPI)

5NTB の概要

• エントリーDOI: 10.2210/pdb5ntb/pdb
• 分子名称: Papain inhibitor, SULFATE ION (3 entities in total)
• 機能のキーワード: streptomyces papain inhibitor, transglutaminase, glutamine donor, streptomyces mobaraensis, protease inhibitor
• 由来する生物種: Streptomyces mobaraensis
• 細胞内の位置: Secreted : P86242
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 24371.60

構造登録者

Schmelz, S.,Juettner, N.E.,Fuchsbauer, H.L.,Scrima, A. (登録日: 2017-04-27, 公開日: 2018-03-07, 最終更新日: 2024-10-09)

主引用文献

Juettner, N.E.,Schmelz, S.,Bogen, J.P.,Happel, D.,Fessner, W.D.,Pfeifer, F.,Fuchsbauer, H.L.,Scrima, A.
Illuminating structure and acyl donor sites of a physiological transglutaminase substrate from Streptomyces mobaraensis.
Protein Sci., 27:910-922, 2018

PubMed Abstract: Transglutaminase from Streptomyces mobaraensis (MTG) has become a powerful tool to covalently and highly specifically link functional amines to glutamine donor sites of therapeutic proteins. However, details regarding the mechanism of substrate recognition and interaction of the enzyme with proteinaceous substrates still remain mostly elusive. We have determined the crystal structure of the Streptomyces papain inhibitory protein (SPI ), a substrate of MTG, to study the influence of various substrate amino acids on positioning glutamine to the active site of MTG. SPI exhibits a rigid, thermo-resistant double-psi-beta-barrel fold that is stabilized by two cysteine bridges. Incorporation of biotin cadaverine identified Gln-6 as the only amine acceptor site on SPI accessible for MTG. Substitution of Lys-7 demonstrated that small and hydrophobic residues in close proximity to Gln-6 favor MTG-mediated modification and are likely to facilitate introduction of the substrate into the front vestibule of MTG. Moreover, exchange of various surface residues of SPI for arginine and glutamate/aspartate outside the glutamine donor region influences the efficiency of modification by MTG. These results suggest the occurrence of charged contact areas between MTG and the acyl donor substrates beyond the front vestibule, and pave the way for protein engineering approaches to improve the properties of artificial MTG-substrates used in biomedical applications.

PubMed: 29430769
DOI: 10.1002/pro.3388

実験手法

X-RAY DIFFRACTION (1.5 Å)