5NQ6

Crystal structure of the inhibited form of the redox-sensitive SufE-like sulfur acceptor CsdE from Escherichia coli at 2.40 Angstrom Resolution

5NQ6 の概要

• エントリーDOI: 10.2210/pdb5nq6/pdb
• 関連するPDBエントリー: 5FT4 5FT5 5FT6 5FT8
• 分子名称: Sulfur acceptor protein CsdE, GLYCEROL, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: sulfur-binding protein, sulfur-acceptor protein, l-cysteine desulfurase, transpersulfuration, sulfur trafficking
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 34385.08

構造登録者

Penya-Soler, E.,Aranda, J.,Lopez-Estepa, M.,Gomez, S.,Garces, F.,Coll, M.,Fernandez, F.J.,Vega, M.C. (登録日: 2017-04-19, 公開日: 2018-03-28, 最終更新日: 2024-10-09)

主引用文献

Pena-Soler, E.,Aranda, J.,Lopez-Estepa, M.,Gomez, S.,Garces, F.,Coll, M.,Fernandez, F.J.,Tunon, I.,Vega, M.C.
Insights into the inhibited form of the redox-sensitive SufE-like sulfur acceptor CsdE.
PLoS ONE, 12:e0186286-e0186286, 2017

PubMed Abstract: Sulfur trafficking in living organisms relies on transpersulfuration reactions consisting in the enzyme-catalyzed transfer of S atoms via activated persulfidic S across protein-protein interfaces. The recent elucidation of the mechanistic basis for transpersulfuration in the CsdA-CsdE model system has paved the way for a better understanding of its role under oxidative stress. Herein we present the crystal structure of the oxidized, inactivated CsdE dimer at 2.4 Å resolution. The structure sheds light into the activation of the Cys61 nucleophile on its way from a solvent-secluded position in free CsdE to a fully extended conformation in the persulfurated CsdA-CsdE complex. Molecular dynamics simulations of available CsdE structures allow to delineate the sequence of conformational changes underwent by CsdE and to pinpoint the key role played by the deprotonation of the Cys61 thiol. The low-energy subunit orientation in the disulfide-bridged CsdE dimer demonstrates the likely physiologic relevance of this oxidative dead-end form of CsdE, suggesting that CsdE could act as a redox sensor in vivo.

PubMed: 29045454
DOI: 10.1371/journal.pone.0186286

実験手法

X-RAY DIFFRACTION (2.4 Å)