5NO7

Crystal Structure of a Xylan-active Lytic Polysaccharide Monooxygenase from Pycnoporus coccineus.

5NO7 の概要

• エントリーDOI: 10.2210/pdb5no7/pdb
• 分子名称: Lytic polysaccharide monooxygenase, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (8 entities in total)
• 機能のキーワード: xylan, lpmo, cellulose, oxydative enzyme, wood, oxidoreductase
• 由来する生物種: Pycnoporus cinnabarinus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 74772.36

構造登録者

Ladeveze, S.,Couturier, M.,Sulzenbacher, G.,Berrin, J.-G. (登録日: 2017-04-11, 公開日: 2018-01-17, 最終更新日: 2024-11-20)

主引用文献

Couturier, M.,Ladeveze, S.,Sulzenbacher, G.,Ciano, L.,Fanuel, M.,Moreau, C.,Villares, A.,Cathala, B.,Chaspoul, F.,Frandsen, K.E.,Labourel, A.,Herpoel-Gimbert, I.,Grisel, S.,Haon, M.,Lenfant, N.,Rogniaux, H.,Ropartz, D.,Davies, G.J.,Rosso, M.N.,Walton, P.H.,Henrissat, B.,Berrin, J.G.
Lytic xylan oxidases from wood-decay fungi unlock biomass degradation.
Nat. Chem. Biol., 14:306-310, 2018

PubMed Abstract: Wood biomass is the most abundant feedstock envisioned for the development of modern biorefineries. However, the cost-effective conversion of this form of biomass into commodity products is limited by its resistance to enzymatic degradation. Here we describe a new family of fungal lytic polysaccharide monooxygenases (LPMOs) prevalent among white-rot and brown-rot basidiomycetes that is active on xylans-a recalcitrant polysaccharide abundant in wood biomass. Two AA14 LPMO members from the white-rot fungus Pycnoporus coccineus substantially increase the efficiency of wood saccharification through oxidative cleavage of highly refractory xylan-coated cellulose fibers. The discovery of this unique enzyme activity advances our knowledge on the degradation of woody biomass in nature and offers an innovative solution for improving enzyme cocktails for biorefinery applications.

PubMed: 29377002
DOI: 10.1038/nchembio.2558

実験手法

X-RAY DIFFRACTION (3.01 Å)