5NO3
RsgA-GDPNP bound to the 30S ribosomal subunit (RsgA assembly intermediate without uS3)
Summary for 5NO3
| Entry DOI | 10.2210/pdb5no3/pdb |
| EMDB information | 3662 |
| Descriptor | 16S ribosomal RNA, 30S ribosomal protein S11, 30S ribosomal protein S12, ... (23 entities in total) |
| Functional Keywords | ribosome |
| Biological source | Escherichia coli (strain K12) More |
| Cellular location | Cytoplasm : P39286 |
| Total number of polymer chains | 20 |
| Total formula weight | 758079.01 |
| Authors | Lopez-Alonso, J.P.,Kaminishi, T.,Kikuchi, T.,Hirata, Y.,Iturrioz, I.,Dhimole, N.,Schedlbauer, A.,Hase, Y.,Goto, S.,Kurita, D.,Muto, A.,Zhou, S.,Naoe, C.,Mills, D.J.,Gil-Carton, D.,Takemoto, C.,Himeno, H.,Fucini, P.,Connell, S.R. (deposition date: 2017-04-10, release date: 2017-05-31, Last modification date: 2024-04-24) |
| Primary citation | Lopez-Alonso, J.P.,Kaminishi, T.,Kikuchi, T.,Hirata, Y.,Iturrioz, I.,Dhimole, N.,Schedlbauer, A.,Hase, Y.,Goto, S.,Kurita, D.,Muto, A.,Zhou, S.,Naoe, C.,Mills, D.J.,Gil-Carton, D.,Takemoto, C.,Himeno, H.,Fucini, P.,Connell, S.R. RsgA couples the maturation state of the 30S ribosomal decoding center to activation of its GTPase pocket. Nucleic Acids Res., 45:6945-6959, 2017 Cited by PubMed Abstract: During 30S ribosomal subunit biogenesis, assembly factors are believed to prevent accumulation of misfolded intermediate states of low free energy that slowly convert into mature 30S subunits, namely, kinetically trapped particles. Among the assembly factors, the circularly permuted GTPase, RsgA, plays a crucial role in the maturation of the 30S decoding center. Here, directed hydroxyl radical probing and single particle cryo-EM are employed to elucidate RsgA΄s mechanism of action. Our results show that RsgA destabilizes the 30S structure, including late binding r-proteins, providing a structural basis for avoiding kinetically trapped assembly intermediates. Moreover, RsgA exploits its distinct GTPase pocket and specific interactions with the 30S to coordinate GTPase activation with the maturation state of the 30S subunit. This coordination validates the architecture of the decoding center and facilitates the timely release of RsgA to control the progression of 30S biogenesis. PubMed: 28482099DOI: 10.1093/nar/gkx324 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (5.16 Å) |
Structure validation
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