5NLM

Complex between a UDP-glucosyltransferase from Polygonum tinctorium capable of glucosylating indoxyl and indoxyl sulfate

5NLM の概要

• エントリーDOI: 10.2210/pdb5nlm/pdb
• 分子名称: indoxyl UDP-glucosyltransferase, 3-SULFOOXY-1H-INDOLE, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: udpglucose:indoxyl glucosyltransferase gt-b ugt indigo, transferase
• 由来する生物種: Persicaria tinctoria
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 104356.59

構造登録者

Welner, D.H.,Hsu, T.,Dueber, J.,Adams, P.D. (登録日: 2017-04-04, 公開日: 2018-01-31, 最終更新日: 2024-01-17)

主引用文献

Hsu, T.M.,Welner, D.H.,Russ, Z.N.,Cervantes, B.,Prathuri, R.L.,Adams, P.D.,Dueber, J.E.
Employing a biochemical protecting group for a sustainable indigo dyeing strategy.
Nat. Chem. Biol., 14:256-261, 2018

PubMed Abstract: Indigo is an ancient dye uniquely capable of producing the signature tones in blue denim; however, the dyeing process requires chemical steps that are environmentally damaging. We describe a sustainable dyeing strategy that not only circumvents the use of toxic reagents for indigo chemical synthesis but also removes the need for a reducing agent for dye solubilization. This strategy utilizes a glucose moiety as a biochemical protecting group to stabilize the reactive indigo precursor indoxyl to form indican, preventing spontaneous oxidation to crystalline indigo during microbial fermentation. Application of a β-glucosidase removes the protecting group from indican, resulting in indigo crystal formation in the cotton fibers. We identified the gene coding for the glucosyltransferase PtUGT1 from the indigo plant Polygonum tinctorium and solved the structure of PtUGT1. Heterologous expression of PtUGT1 in Escherichia coli supported high indican conversion, and biosynthesized indican was used to dye cotton swatches and a garment.

PubMed: 29309053
DOI: 10.1038/nchembio.2552

実験手法

X-RAY DIFFRACTION (2.14 Å)