5NJS

Mix-and-diffuse serial synchrotron crystallography: structure of N,N',N''-Triacetylchitotriose bound to Lysozyme with 50s time-delay, phased with 1HEW

5NJS の概要

• エントリーDOI: 10.2210/pdb5njs/pdb
• 関連するPDBエントリー: 5NJP 5NJQ
• 関連するBIRD辞書のPRD_ID: PRD_900017
• 分子名称: Lysozyme C, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: competitive inhibitor glycoside hydrolase, hydrolase
• 由来する生物種: Gallus gallus (Chicken)
• 細胞内の位置: Secreted: P00698
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15088.10

構造登録者

Oberthuer, D.,Meents, A.,Beyerlein, K.R.,Chapman, H.N.,Lieseke, J. (登録日: 2017-03-29, 公開日: 2017-10-18, 最終更新日: 2024-11-13)

主引用文献

Beyerlein, K.R.,Dierksmeyer, D.,Mariani, V.,Kuhn, M.,Sarrou, I.,Ottaviano, A.,Awel, S.,Knoska, J.,Fuglerud, S.,Jonsson, O.,Stern, S.,Wiedorn, M.O.,Yefanov, O.,Adriano, L.,Bean, R.,Burkhardt, A.,Fischer, P.,Heymann, M.,Horke, D.A.,Jungnickel, K.E.J.,Kovaleva, E.,Lorbeer, O.,Metz, M.,Meyer, J.,Morgan, A.,Pande, K.,Panneerselvam, S.,Seuring, C.,Tolstikova, A.,Lieske, J.,Aplin, S.,Roessle, M.,White, T.A.,Chapman, H.N.,Meents, A.,Oberthuer, D.
Mix-and-diffuse serial synchrotron crystallography.
IUCrJ, 4:769-777, 2017

PubMed Abstract: Unravelling the interaction of biological macromolecules with ligands and substrates at high spatial and temporal resolution remains a major challenge in structural biology. The development of serial crystallography methods at X-ray free-electron lasers and subsequently at synchrotron light sources allows new approaches to tackle this challenge. Here, a new polyimide tape drive designed for mix-and-diffuse serial crystallography experiments is reported. The structure of lysozyme bound by the competitive inhibitor chitotriose was determined using this device in combination with microfluidic mixers. The electron densities obtained from mixing times of 2 and 50 s show clear binding of chitotriose to the enzyme at a high level of detail. The success of this approach shows the potential for high-throughput drug screening and even structural enzymology on short timescales at bright synchrotron light sources.

PubMed: 29123679
DOI: 10.1107/S2052252517013124

実験手法

X-RAY DIFFRACTION (1.7 Å)