5NJP

Mix-and-diffuse serial synchrotron crystallography: structure of N,N',N''-Triacetylchitotriose bound to Lysozyme with 1s time-delay, phased with 1HEW

Summary for 5NJP

• Entry DOI: 10.2210/pdb5njp/pdb
• Related PRD ID: PRD_900017
• Descriptor: Lysozyme C, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, CHLORIDE ION, ... (5 entities in total)
• Functional Keywords: competitive inhibitor glycoside hydrolase, hydrolase
• Biological source: Gallus gallus (Chicken)
• Cellular location: Secreted: P00698
• Total number of polymer chains: 1
• Total formula weight: 15052.65

Authors

Oberthuer, D.,Meents, A.,Beyerlein, K.R.,Chapman, H.N.,Lieseke, J. (deposition date: 2017-03-29, release date: 2017-10-18, Last modification date: 2024-10-23)

Primary citation

Beyerlein, K.R.,Dierksmeyer, D.,Mariani, V.,Kuhn, M.,Sarrou, I.,Ottaviano, A.,Awel, S.,Knoska, J.,Fuglerud, S.,Jonsson, O.,Stern, S.,Wiedorn, M.O.,Yefanov, O.,Adriano, L.,Bean, R.,Burkhardt, A.,Fischer, P.,Heymann, M.,Horke, D.A.,Jungnickel, K.E.J.,Kovaleva, E.,Lorbeer, O.,Metz, M.,Meyer, J.,Morgan, A.,Pande, K.,Panneerselvam, S.,Seuring, C.,Tolstikova, A.,Lieske, J.,Aplin, S.,Roessle, M.,White, T.A.,Chapman, H.N.,Meents, A.,Oberthuer, D.
Mix-and-diffuse serial synchrotron crystallography.
IUCrJ, 4:769-777, 2017

PubMed Abstract: Unravelling the interaction of biological macromolecules with ligands and substrates at high spatial and temporal resolution remains a major challenge in structural biology. The development of serial crystallography methods at X-ray free-electron lasers and subsequently at synchrotron light sources allows new approaches to tackle this challenge. Here, a new polyimide tape drive designed for mix-and-diffuse serial crystallography experiments is reported. The structure of lysozyme bound by the competitive inhibitor chitotriose was determined using this device in combination with microfluidic mixers. The electron densities obtained from mixing times of 2 and 50 s show clear binding of chitotriose to the enzyme at a high level of detail. The success of this approach shows the potential for high-throughput drug screening and even structural enzymology on short timescales at bright synchrotron light sources.

PubMed: 29123679
DOI: 10.1107/S2052252517013124

Experimental method

X-RAY DIFFRACTION (1.7 Å)