5NIP

An i-motif containing the neutral cytidine protonated analogue pseudoisocytidine

Summary for 5NIP

• Entry DOI: 10.2210/pdb5nip/pdb
• NMR Information: BMRB: 34118
• Descriptor: DNA (5'-D(*TP*(DCP)P*CP*GP*TP*TP*TP*CP*(PSC)P*GP*T)-3') (1 entity in total)
• Functional Keywords: dna
• Biological source: synthetic construct
• Total number of polymer chains: 2
• Total formula weight: 6582.29

Authors

Mir, B.,Soles, X.,Gonzalez, C.,Escaja, N. (deposition date: 2017-03-24, release date: 2017-06-28, Last modification date: 2024-05-15)

Primary citation

Mir, B.,Soles, X.,Gonzalez, C.,Escaja, N.
The effect of the neutral cytidine protonated analogue pseudoisocytidine on the stability of i-motif structures.
Sci Rep, 7:2772-2772, 2017

PubMed Abstract: Incorporation of pseudoisocytidine (psC), a neutral analogue of protonated cytidine, in i-motifs has been studied by spectroscopic methods. Our results show that neutral psC:C base pairs can stabilize i-motifs at neutral pH, but the stabilization only occurs when psC:C base pairs are located at the ends of intercalated C:C stacks. When psC occupies central positions, the resulting i-motifs are only observed at low pH and psC:C or psC:psC hemiprotonated base pairs are formed instead of their neutral analogs. Overall, our results suggest that positively charged base pairs are necessary to stabilize this non-canonical DNA structure.

PubMed: 28584239
DOI: 10.1038/s41598-017-02723-y

Experimental method

SOLUTION NMR