5NIP

An i-motif containing the neutral cytidine protonated analogue pseudoisocytidine

5NIP の概要

• エントリーDOI: 10.2210/pdb5nip/pdb
• NMR情報: BMRB: 34118
• 分子名称: DNA (5'-D(*TP*(DCP)P*CP*GP*TP*TP*TP*CP*(PSC)P*GP*T)-3') (1 entity in total)
• 機能のキーワード: dna
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 6582.29

構造登録者

Mir, B.,Soles, X.,Gonzalez, C.,Escaja, N. (登録日: 2017-03-24, 公開日: 2017-06-28, 最終更新日: 2024-05-15)

主引用文献

Mir, B.,Soles, X.,Gonzalez, C.,Escaja, N.
The effect of the neutral cytidine protonated analogue pseudoisocytidine on the stability of i-motif structures.
Sci Rep, 7:2772-2772, 2017

PubMed Abstract: Incorporation of pseudoisocytidine (psC), a neutral analogue of protonated cytidine, in i-motifs has been studied by spectroscopic methods. Our results show that neutral psC:C base pairs can stabilize i-motifs at neutral pH, but the stabilization only occurs when psC:C base pairs are located at the ends of intercalated C:C stacks. When psC occupies central positions, the resulting i-motifs are only observed at low pH and psC:C or psC:psC hemiprotonated base pairs are formed instead of their neutral analogs. Overall, our results suggest that positively charged base pairs are necessary to stabilize this non-canonical DNA structure.

PubMed: 28584239
DOI: 10.1038/s41598-017-02723-y

実験手法

SOLUTION NMR