5NG4
Human CEP135 parallel dimeric coiled coil 82-144
Summary for 5NG4
| Entry DOI | 10.2210/pdb5ng4/pdb |
| Descriptor | Centrosomal protein of 135 kDa (2 entities in total) |
| Functional Keywords | coiled coil, cep135, centrosome, disulfide bridges, microtubule-binding protein |
| Biological source | Homo sapiens (Human) |
| Total number of polymer chains | 2 |
| Total formula weight | 15455.79 |
| Authors | Kraatz, S.H.W.,Bianchi, S.,Steinmetz, M.O. (deposition date: 2017-03-16, release date: 2018-05-02, Last modification date: 2018-10-03) |
| Primary citation | Kraatz, S.H.W.,Bianchi, S.,Steinmetz, M.O. Combinatorial use of disulfide bridges and native sulfur-SAD phasing for rapid structure determination of coiled-coils. Biosci. Rep., 38:-, 2018 Cited by PubMed Abstract: Coiled-coils are ubiquitous protein-protein interaction motifs found in many eukaryotic proteins. The elongated, flexible and often irregular nature of coiled-coils together with their tendency to form fibrous arrangements in crystals imposes challenges on solving the phase problem by molecular replacement. Here, we report the successful combinatorial use of native and rational engineered disulfide bridges together with sulfur-SAD phasing as a powerful tool to stabilize and solve the structure of coiled-coil domains in a straightforward manner. Our study is a key example of how modern sulfur SAD combined with mutagenesis can help to advance and simplify the structural study of challenging coiled-coil domains by X-ray crystallography. PubMed: 30135143DOI: 10.1042/BSR20181073 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.14 Å) |
Structure validation
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