5NG4

Human CEP135 parallel dimeric coiled coil 82-144

5NG4 の概要

• エントリーDOI: 10.2210/pdb5ng4/pdb
• 分子名称: Centrosomal protein of 135 kDa (2 entities in total)
• 機能のキーワード: coiled coil, cep135, centrosome, disulfide bridges, microtubule-binding protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 15455.79

構造登録者

Kraatz, S.H.W.,Bianchi, S.,Steinmetz, M.O. (登録日: 2017-03-16, 公開日: 2018-05-02, 最終更新日: 2018-10-03)

主引用文献

Kraatz, S.H.W.,Bianchi, S.,Steinmetz, M.O.
Combinatorial use of disulfide bridges and native sulfur-SAD phasing for rapid structure determination of coiled-coils.
Biosci. Rep., 38:-, 2018

PubMed Abstract: Coiled-coils are ubiquitous protein-protein interaction motifs found in many eukaryotic proteins. The elongated, flexible and often irregular nature of coiled-coils together with their tendency to form fibrous arrangements in crystals imposes challenges on solving the phase problem by molecular replacement. Here, we report the successful combinatorial use of native and rational engineered disulfide bridges together with sulfur-SAD phasing as a powerful tool to stabilize and solve the structure of coiled-coil domains in a straightforward manner. Our study is a key example of how modern sulfur SAD combined with mutagenesis can help to advance and simplify the structural study of challenging coiled-coil domains by X-ray crystallography.

PubMed: 30135143
DOI: 10.1042/BSR20181073

実験手法

X-RAY DIFFRACTION (2.14 Å)