5NFD

Antiparallel monomeric coiled coil of Kif21A

Summary for 5NFD

• Entry DOI: 10.2210/pdb5nfd/pdb
• Descriptor: Kinesin-like protein KIF21A (2 entities in total)
• Functional Keywords: coiled coil, kinesin, motor protein
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 2
• Total formula weight: 18929.29

Authors

Kraatz, S.H.W.,Bianchi, S.,Steinmetz, M.O. (deposition date: 2017-03-13, release date: 2018-05-02, Last modification date: 2024-11-13)

Primary citation

Kraatz, S.H.W.,Bianchi, S.,Steinmetz, M.O.
Combinatorial use of disulfide bridges and native sulfur-SAD phasing for rapid structure determination of coiled-coils.
Biosci. Rep., 38:-, 2018

PubMed Abstract: Coiled-coils are ubiquitous protein-protein interaction motifs found in many eukaryotic proteins. The elongated, flexible and often irregular nature of coiled-coils together with their tendency to form fibrous arrangements in crystals imposes challenges on solving the phase problem by molecular replacement. Here, we report the successful combinatorial use of native and rational engineered disulfide bridges together with sulfur-SAD phasing as a powerful tool to stabilize and solve the structure of coiled-coil domains in a straightforward manner. Our study is a key example of how modern sulfur SAD combined with mutagenesis can help to advance and simplify the structural study of challenging coiled-coil domains by X-ray crystallography.

PubMed: 30135143
DOI: 10.1042/BSR20181073

Experimental method

X-RAY DIFFRACTION (2.18 Å)