5NCE
Structure of PsDef1 defensin from Pinus sylvestris
Summary for 5NCE
| Entry DOI | 10.2210/pdb5nce/pdb |
| NMR Information | BMRB: 34113 |
| Descriptor | Defensin-1 (1 entity in total) |
| Functional Keywords | defensin, plant defensin, plant protein, scotts pine defensin, conformational dynamics, antibacterial activity, amylase inhibition |
| Biological source | Pinus sylvestris (Scots pine) |
| Cellular location | Secreted : A4L7R7 |
| Total number of polymer chains | 1 |
| Total formula weight | 5611.57 |
| Authors | Khairutdinov, B.I.,Ermakova, E.A.,Bessolitsyna, E.K.,Toporkova, Y.Y.,Tarasova, N.B.,Kovaleva, V.,Zuev, Y.F.,Nesmelova, I.V. (deposition date: 2017-03-03, release date: 2017-06-07, Last modification date: 2024-11-06) |
| Primary citation | Khairutdinov, B.I.,Ermakova, E.A.,Yusypovych, Y.M.,Bessolicina, E.K.,Tarasova, N.B.,Toporkova, Y.Y.,Kovaleva, V.,Zuev, Y.F.,Nesmelova, I.V. NMR structure, conformational dynamics, and biological activity of PsDef1 defensin from Pinus sylvestris. Biochim. Biophys. Acta, 1865:1085-1094, 2017 Cited by PubMed Abstract: Plants have developed a complex defense response system against pests and pathogens. Defensins, produced by plants as part of their innate immune response, form the family of small, basic, cysteine-rich proteins with activity primarily directed against fungal pathogens. In addition, plant defensins can show antibacterial activity and protease and insect amylase inhibitory activities. However, in gymnosperms, only antifungal activity of defensins has been described thus far. Here, we report antibacterial and insect α-amylase inhibition activities for defensin PsDef1 from P. sylvestris, the first defensin from gymnosperms with a broad range of biological activities described. We also report the solution NMR structure of PsDef1 and its dynamics properties assessed by a combination of experimental NMR and computational techniques. Collectively, our data provide an insight into structure, dynamics, and functional properties of PsDef1 that could be common between defensins from this taxonomic group. PubMed: 28528214DOI: 10.1016/j.bbapap.2017.05.012 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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