5NCE

Structure of PsDef1 defensin from Pinus sylvestris

5NCE の概要

• エントリーDOI: 10.2210/pdb5nce/pdb
• NMR情報: BMRB: 34113
• 分子名称: Defensin-1 (1 entity in total)
• 機能のキーワード: defensin, plant defensin, plant protein, scotts pine defensin, conformational dynamics, antibacterial activity, amylase inhibition
• 由来する生物種: Pinus sylvestris (Scots pine)
• 細胞内の位置: Secreted : A4L7R7
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5611.57

構造登録者

Khairutdinov, B.I.,Ermakova, E.A.,Bessolitsyna, E.K.,Toporkova, Y.Y.,Tarasova, N.B.,Kovaleva, V.,Zuev, Y.F.,Nesmelova, I.V. (登録日: 2017-03-03, 公開日: 2017-06-07, 最終更新日: 2024-11-06)

主引用文献

Khairutdinov, B.I.,Ermakova, E.A.,Yusypovych, Y.M.,Bessolicina, E.K.,Tarasova, N.B.,Toporkova, Y.Y.,Kovaleva, V.,Zuev, Y.F.,Nesmelova, I.V.
NMR structure, conformational dynamics, and biological activity of PsDef1 defensin from Pinus sylvestris.
Biochim. Biophys. Acta, 1865:1085-1094, 2017

PubMed Abstract: Plants have developed a complex defense response system against pests and pathogens. Defensins, produced by plants as part of their innate immune response, form the family of small, basic, cysteine-rich proteins with activity primarily directed against fungal pathogens. In addition, plant defensins can show antibacterial activity and protease and insect amylase inhibitory activities. However, in gymnosperms, only antifungal activity of defensins has been described thus far. Here, we report antibacterial and insect α-amylase inhibition activities for defensin PsDef1 from P. sylvestris, the first defensin from gymnosperms with a broad range of biological activities described. We also report the solution NMR structure of PsDef1 and its dynamics properties assessed by a combination of experimental NMR and computational techniques. Collectively, our data provide an insight into structure, dynamics, and functional properties of PsDef1 that could be common between defensins from this taxonomic group.

PubMed: 28528214
DOI: 10.1016/j.bbapap.2017.05.012

実験手法

SOLUTION NMR