5NBO

Bacteroides ovatus mixed linkage glucan PUL (MLGUL) GH16

5NBO の概要

• エントリーDOI: 10.2210/pdb5nbo/pdb
• 分子名称: Glycosyl hydrolase family 16, 1,2-ETHANEDIOL (3 entities in total)
• 機能のキーワード: glycoside hydrolase, gh16, hydrolase
• 由来する生物種: Bacteroides ovatus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 61428.09

構造登録者

Hemsworth, G.R.,Tamura, K.,Dejean, G.,Rogers, T.E.,Pudlo, N.A.,Urs, K.,Jain, N.,Martens, E.C.,Brumer, H.,Davies, G.J. (登録日: 2017-03-02, 公開日: 2017-10-25, 最終更新日: 2024-01-17)

主引用文献

Tamura, K.,Hemsworth, G.R.,Dejean, G.,Rogers, T.E.,Pudlo, N.A.,Urs, K.,Jain, N.,Davies, G.J.,Martens, E.C.,Brumer, H.
Molecular Mechanism by which Prominent Human Gut Bacteroidetes Utilize Mixed-Linkage Beta-Glucans, Major Health-Promoting Cereal Polysaccharides.
Cell Rep, 21:417-430, 2017

PubMed Abstract: Microbial utilization of complex polysaccharides is a major driving force in shaping the composition of the human gut microbiota. There is a growing appreciation that finely tuned polysaccharide utilization loci enable ubiquitous gut Bacteroidetes to thrive on the plethora of complex polysaccharides that constitute "dietary fiber." Mixed-linkage β(1,3)/β(1,4)-glucans (MLGs) are a key family of plant cell wall polysaccharides with recognized health benefits but whose mechanism of utilization has remained unclear. Here, we provide molecular insight into the function of an archetypal MLG utilization locus (MLGUL) through a combination of biochemistry, enzymology, structural biology, and microbiology. Comparative genomics coupled with growth studies demonstrated further that syntenic MLGULs serve as genetic markers for MLG catabolism across commensal gut bacteria. In turn, we surveyed human gut metagenomes to reveal that MLGULs are ubiquitous in human populations globally, which underscores the importance of gut microbial metabolism of MLG as a common cereal polysaccharide.

PubMed: 29020628
DOI: 10.1016/j.celrep.2017.09.049

実験手法

X-RAY DIFFRACTION (1.8 Å)