5N97

Structure of the C. crescentus S-layer

5N97 の概要

• エントリーDOI: 10.2210/pdb5n97/pdb
• EMDBエントリー: 3604
• 分子名称: S-layer protein rsaA, CALCIUM ION (2 entities in total)
• 機能のキーワード: s-layer, sub-tomogram averaging, bacteria, cell surface, caulobacter, structural protein
• 由来する生物種: Caulobacter crescentus NA1000
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 443524.72

構造登録者

Bharat, T.A.,Hagen, W.J.,Briggs, J.A.,Lowe, J. (登録日: 2017-02-24, 公開日: 2017-04-19, 最終更新日: 2024-05-15)

主引用文献

Bharat, T.A.M.,Kureisaite-Ciziene, D.,Hardy, G.G.,Yu, E.W.,Devant, J.M.,Hagen, W.J.H.,Brun, Y.V.,Briggs, J.A.G.,Lowe, J.
Structure of the hexagonal surface layer on Caulobacter crescentus cells.
Nat Microbiol, 2:17059-17059, 2017

PubMed Abstract: Many prokaryotic cells are encapsulated by a surface layer (S-layer) consisting of repeating units of S-layer proteins. S-layer proteins are a diverse class of molecules found in Gram-positive and Gram-negative bacteria and most archaea. S-layers protect cells from the outside, provide mechanical stability and also play roles in pathogenicity. In situ structural information about this highly abundant class of proteins is scarce, so atomic details of how S-layers are arranged on the surface of cells have remained elusive. Here, using purified Caulobacter crescentus' sole S-layer protein RsaA, we obtained a 2.7 Å X-ray structure that shows the hexameric S-layer lattice. We also solved a 7.4 Å structure of the S-layer through electron cryotomography and sub-tomogram averaging of cell stalks. The X-ray structure was docked unambiguously into the electron cryotomography map, resulting in a pseudo-atomic-level description of the in vivo S-layer, which agrees completely with the atomic X-ray lattice model. The cellular S-layer atomic structure shows that the S-layer is porous, with a largest gap dimension of 27 Å, and is stabilized by multiple Ca ions bound near the interfaces. This study spans different spatial scales from atoms to cells by combining X-ray crystallography with electron cryotomography and sub-nanometre-resolution sub-tomogram averaging.

PubMed: 28418382
DOI: 10.1038/nmicrobiol.2017.59

実験手法

ELECTRON MICROSCOPY (7.4 Å)