5N35

Gadolinium phased PBP2 (SSO6202) at 2.2 Ang

5N35 の概要

• エントリーDOI: 10.2210/pdb5n35/pdb
• 関連するPDBエントリー: 5N41
• 分子名称: PolB1 Binding Protein 2 (PBP2), GADOLINIUM ATOM, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: polb1 binding protein 2, archaeal dna polymerase holoenzyme, protein binding, polymerase binding protein
• 由来する生物種: Sulfolobus solfataricus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9340.83

構造登録者

Yan, J.,Beattie, T.R.,Rojas, A.L.,Schermerhorn, K.,Gristwood, T.,Trinidad, J.C.,Albers, S.V.,Roversi, P.,Gardner, A.F.,Abrescia, N.G.A.,Bell, S.D. (登録日: 2017-02-08, 公開日: 2017-05-17, 最終更新日: 2024-10-16)

主引用文献

Yan, J.,Beattie, T.R.,Rojas, A.L.,Schermerhorn, K.,Gristwood, T.,Trinidad, J.C.,Albers, S.V.,Roversi, P.,Gardner, A.F.,Abrescia, N.G.A.,Bell, S.D.
Identification and characterization of a heterotrimeric archaeal DNA polymerase holoenzyme.
Nat Commun, 8:15075-15075, 2017

PubMed Abstract: Since their initial characterization over 30 years ago, it has been believed that the archaeal B-family DNA polymerases are single-subunit enzymes. This contrasts with the multi-subunit B-family replicative polymerases of eukaryotes. Here we reveal that the highly studied PolB1 from Sulfolobus solfataricus exists as a heterotrimeric complex in cell extracts. Two small subunits, PBP1 and PBP2, associate with distinct surfaces of the larger catalytic subunit and influence the enzymatic properties of the DNA polymerase. Thus, multi-subunit replicative DNA polymerase holoenzymes are present in all three domains of life. We reveal the architecture of the assembly by a combination of cross-linking coupled with mass spectrometry, X-ray crystallography and single-particle electron microscopy. The small subunits stabilize the holoenzyme assembly and the acidic tail of one small subunit mitigates the ability of the enzyme to perform strand-displacement synthesis, with important implications for lagging strand DNA synthesis.

PubMed: 28462924
DOI: 10.1038/ncomms15075

実験手法

X-RAY DIFFRACTION (2.24 Å)