5N2C

Crystal structure of the peptidoglycan-associated lipoprotein from Burkholderia cenocepacia

Summary for 5N2C

• Entry DOI: 10.2210/pdb5n2c/pdb
• Descriptor: Putative OmpA family lipoprotein, 1,2-ETHANEDIOL, 3-CYCLOHEXYL-1-PROPYLSULFONIC ACID, ... (6 entities in total)
• Functional Keywords: outer membrane protein a like domain, lipoprotein, antigen, transport protein
• Biological source: Burkholderia cenocepacia J2315
• Total number of polymer chains: 3
• Total formula weight: 63177.74

Authors

Matterazzo, E.,Bolognesi, M.,Gourlay, L.J. (deposition date: 2017-02-07, release date: 2017-07-26, Last modification date: 2024-01-17)

Primary citation

Capelli, R.,Matterazzo, E.,Amabili, M.,Peri, C.,Gori, A.,Gagni, P.,Chiari, M.,Lertmemongkolchai, G.,Cretich, M.,Bolognesi, M.,Colombo, G.,Gourlay, L.J.
Designing Probes for Immunodiagnostics: Structural Insights into an Epitope Targeting Burkholderia Infections.
ACS Infect Dis, 3:736-743, 2017

PubMed Abstract: Structure-based epitope prediction drives the design of diagnostic peptidic probes to reveal specific antibodies elicited in response to infections. We previously identified a highly immunoreactive epitope from the peptidoglycan-associated lipoprotein (Pal) antigen from Burkholderia pseudomallei, which could also diagnose Burkholderia cepacia infections. Here, considering the high phylogenetic conservation within Burkholderia species, we ask whether cross-reactivity can be reciprocally displayed by the synthetic epitope from B. cenocepacia. We perform comparative analyses of the conformational preferences and diagnostic performances of the corresponding epitopes from the two Burkholderia species when presented in the context of the full-length proteins or as isolated peptides. The effects of conformation on the diagnostic potential and cross-reactivity of Pal peptide epitopes are rationalized on the basis of the 1.8 Å crystal structure of B. cenocepacia Pal and through computational analyses. Our results are discussed in the context of designing new diagnostic molecules for the early detection of infectious diseases.

PubMed: 28707874
DOI: 10.1021/acsinfecdis.7b00080

Experimental method

X-RAY DIFFRACTION (1.8 Å)